Quantification and characterization of high‐affinity membrane receptors for tumor necrosis factor on human leukemic cell lines
Quantification and characterization of high‐affinity membrane receptors for tumor necrosis factor on human leukemic cell lines
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人白血病细胞系肿瘤坏死因子高亲和力膜受体的定量和表征
DOI：
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发表时间：
1986
影响因子：
6.4
通讯作者：
K. Pfizenmaier
中科院分区：
文献类型：
--
作者：
P. Scheurich;U. Üçer;M. Krönke;K. Pfizenmaier
在对TNF-α生长抑制活性敏感性不同的各种人白血病细胞系上测定TNF-α特异性膜受体的表达。125 M标记TNF-α的结合研究表明，在8/10个细胞系中特异性结合，这些不同细胞系结合的TNF-α量存在约10倍差异。TNF结合的Scatchard分析显示，U937和K562上存在高亲和力膜受体（Kd 1.5 × 10−10 M）和约3，000个结合位点/细胞，分别代表2种TNF敏感性高和低的细胞系。受体结合的125 I-TNF-α的二琥珀酰亚胺辛二酸酯交联和U937或K562细胞膜制备物的SDS-PAGE表明，表观分子量为76 kDa的单一受体蛋白。TNF-α结合能力与体外生长抑制的比较提供了证据，表明在受体表达水平和受体后水平确定了对TNF-α的敏感性。IFN-γ强烈增强了TNF-α介导的3种敏感细胞系的生长抑制，但对其他7种TNF敏感性很低或不敏感的白血病细胞系没有影响。在TNF敏感性的这种增强与IFN-γ介导的TNF-细胞膜受体的增加之间没有发现相关性，表明IFN-γ主要在TNF-结合远端发挥其协同作用。
The expression of specific membrane receptors for TNF‐aipha was determined on various human leukemic cell lines differing in their sensitivity to the growth‐inhibitory activity of TNF‐alpha. Binding studies with 125Mabelled TNF‐alpha indicated specific binding in 8/10 cell lines with approximately 10‐fold differences in the quantity of TNF‐alpha bound by these distinct cell lines. Scatchard analyses of TNF‐binding revealed the existence of high‐affinity membrane receptors (Kd 1.5 × 10−10 M) and approximately 3,000 binding sites/cell on both U937 and K562, representing 2 cell lines with high and low TNF sensitivity, respectively. Disuccin‐imidyl‐suberate cross‐linking of receptor‐bound 125I‐TNF‐alpha and SDS‐PAGE of membrane preparations of either U937 or K562 cells suggest a single receptor protein with an apparent molecular weight of 76 kDa. Comparison of the TNF‐alpha binding capacity versus in vitro growth inhibition provides evidence that sensitivity to TNF‐alpha is determined both at the level of receptor expression and at a post‐receptor level. IFN‐gamma strongly enhanced the TNF‐alpha‐mediated growth inhibition of 3 sensitive cell lines, but had no effect on 7 other leukemic cell lines with little or no TNF sensitivity. No correlation was found between this enhancement of TNF sensitivity and the IFN‐gamma‐mediated increase in TNF‐cell membrane receptors, suggesting that IFN‐gamma predominantly exerts its synergistic effect distal to TNF‐binding.
DOI：
10.1073/pnas.82.22.7626
发表时间：
1985-01-01
影响因子：
11.1
作者：
TSUJIMOTO, M;YIP, YK;VILCEK, J
通讯作者：
VILCEK, J
DOI：
10.1073/pnas.82.24.8667
发表时间：
1985-12-01
影响因子：
11.1
作者：
GAMBLE, JR;HARLAN, JM;VADAS, MA
通讯作者：
VADAS, MA
肿瘤坏死因子的细胞周期特异性作用。
影响因子：
11.2
作者：
Darzynkiewicz,Z;Williamson,B;Carswell,EA;Old,LJ
通讯作者：
Old,LJ