In vitro antifungal susceptibility of Candida spp. oral isolates from HIV-positive patients and control individuals
In vitro antifungal susceptibility of Candida spp. oral isolates from HIV-positive patients and control individuals
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DOI：
10.1590/s1806-83242011005000001
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发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Koga-Ito, Cristiane Yumi
中科院分区：
文献类型：
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作者：
Brito, Graziella Nuernberg Back;Inocêncio, Aline Cássia;Koga-Ito, Cristiane Yumi
口咽念珠菌病是hiv阳性患者中最常见的真菌感染。这种情况可以用全身或局部抗真菌药物治疗；治疗通常以临床资料为基础，以经验为依据。体外抗真菌敏感性的知识是重要的，以确定正确的治疗指导真菌感染的治疗。因此，本研究的目的是确定从hiv阳性患者和对照个体中分离的口腔念珠菌的抗真菌敏感性。两性霉素B、氟康唑、氟胞嘧啶、制霉菌素和酮康唑按照临床与实验室标准协会（CLSI）提出的微量稀释法进行检测；记录最小抑菌浓度（MIC）值。从hiv阳性患者中分离出71株念珠菌，其中白色念珠菌59株，热带念珠菌9株，光念珠菌1株，吉列蒙地念珠菌1株，克鲁塞念珠菌1株。从11份白色念珠菌和4份热带念珠菌的对照样本中，共分离出15株念珠菌。结果表明，所测试的抗真菌药物对两组的大多数分离株都有良好的活性；然而，观察到不同菌株的MIC值存在差异。
Oropharyngeal candidiasis is the most common fungal infection among HIV-positive patients. This condition can be treated with either systemic or topical antifungal agents; treatments are usually indicated empirically on the basis of clinical data. The knowledge of in vitro antifungal susceptibility is important to determine correct therapeutic guides for the treatment of fungal infections. Therefore, the objective of this study was to determine the antifungal susceptibility profile of oral Candida isolates from HIV-positive patients and control individuals. Amphotericin B, fluconazole, flucytosine, nystatin and ketoconazole were tested according to the methodology of microdilution proposed by the Clinical and Laboratory Standards Institute (CLSI); results were recorded in values of minimal inhibitory concentration (MIC). A total of 71 Candida isolates from HIV-positive patients were examined with the following species represented: C. albicans (59), C. tropicalis (9), C. glabrata (1), C. guilliermondii (1) and C. krusei (1). A total of 15 Candida isolates were evaluated from control individuals comprised of 11 C. albicans and 4 C. tropicalis samples. Our results demonstrated that the tested antifungal agents showed good activity for most isolates from both groups; however, variability in MIC values among isolates was observed.