SULFURIC ACID IN DESMARESTIA
SULFURIC ACID IN DESMARESTIA
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Desmarestia 中的硫酸
DOI：
10.2307/1539096
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
C. Bovell
中科院分区：
文献类型：
--
作者：
R. Eppley;C. Bovell
1. 褐皮菊和苍草菊液泡中积聚了鲜艳的甲酚蓝，积聚的染料呈紫色，说明液泡汁液的pH值小于1.0或大于7.5。而这两种褐藻表达液的pH值分别为1.0以下和2.0左右。树液的低pH值会损伤外细胞膜，而在这种低pH值下，组织匀浆不会还原亚甲基蓝。氰化钠、二硝基苯酚、碘乙酸盐和对氯喹苯甲酸盐诱导细胞释放酸，其中钾，通常是棕色藻细胞中最丰富的阳离子，大部分被氢所取代。在D. munda中，氢占细胞内阳离子含量的75%。组织钠被大量结合，对细胞阳离子含量贡献不大。对这些数据的最简单的解释是，这种酸位于Desmarestia细胞的液泡内。
1. Brilliant cresyl blue accumulates in the vacuoles of Desmarestia munda and D. herbacea and the accumulated dye appears purple, indicating that the pH of the vacuolar sap is less than 1.0 or greater than 7.5. However, the expressed saps of these two brown algae have pH 1.0 or less and about 2.0, respectively. The outer cell membranes are injured by the low pH of the sap and methylene blue is not reduced by tissue homogenates at such low pH values.2. Sodium cyanide, dinitrophenol, iodoacetate, and p-chloromercuribenzoate induce the release of acid from the cells, in which potassium, normally the cation most abundant in brown algal cells, is largely replaced by hydrogen. In D. munda hydrogen accounts for 75 per cent of the intracellular cation content. Tissue sodium is largely bound and contributes little to the cellular cation content.3. The simplest interpretation of these data is that the acid is localized within the vacuoles of Desmarestia cells.