The role of calcium-dependent protein kinase in hydrogen peroxide, nitric oxide and ABA-dependent cold acclimation.
The role of calcium-dependent protein kinase in hydrogen peroxide, nitric oxide and ABA-dependent cold acclimation.
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钙依赖性蛋白激酶在过氧化氢、一氧化氮和ABA依赖性冷驯化中的作用
DOI：
10.1093/jxb/ery212
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发表时间：
2018-07-18
影响因子：
6.9
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Lv X;Li H;Chen X;Xiang X;Guo Z;Yu J;Zhou Y
沉默番茄中的钙依赖性蛋白激酶CPK 27降低了冷诱导的阿坝、H2 O2和NO的产生，以及降低了丝裂原活化蛋白激酶的活化，从而损害了冷驯化。冷驯化诱导的植物耐冷性与活性氧（ROS）、一氧化氮（NO）和丝裂原活化蛋白激酶（MPKs）的产生有关。在这里，我们假设，钙依赖性蛋白激酶（CPKs）诱导ROS，NO和MPKs之间的串扰，导致脱落酸（阿坝）信号在植物适应冷胁迫的激活。结果表明，冷锻炼显著提高了番茄CPK 27的转录水平，同时沿着阿坝的合成。CPK 27的沉默损害了驯化诱导的耐冷性，质外体中过氧化氢（H2 O2）的产生，NO和阿坝的积累，以及MPK 1/2的激活。H2 O2、NO和MPK 1/2之间的相互作用有助于H2 O2和NO的稳态、MPK 1/2的激活和耐寒性。阿坝对CPK 27诱导的耐寒性、H2 O2和NO的产生以及MPK 1/2的激活也是至关重要的。这些结果表明，CPK 27在冷适应过程中可能通过激活ROS和NO以及MPK 1/2的产生而作为阿坝产生的正调节因子发挥作用。
Silencing the calcium-dependent protein kinase CPK27 in tomato decreases cold-induced ABA, H2O2 and NO production, as well as decreasing activation of mitogen-activated protein kinase, compromising cold acclimation. Cold acclimation-induced cold tolerance is associated with the generation of reactive oxygen species (ROS), nitric oxide (NO), and mitogen-activated protein kinases (MPKs) in plants. Here, we hypothesized that calcium-dependent protein kinases (CPKs) induce a crosstalk among ROS, NO, and MPKs, leading to the activation of abscisic acid (ABA) signaling in plant adaptation to cold stress. Results showed that cold acclimation significantly increased the transcript levels of CPK27 along with the biosynthesis of ABA in tomato (Solanum lycopersicum). Silencing of CPK27 compromised acclimation-induced cold tolerance, generation of hydrogen peroxide (H2O2) in the apoplast, NO and ABA accumulation, and the activation of MPK1/2. Crosstalk among H2O2, NO, and MPK1/2 contributes to the homeostasis of H2O2 and NO, activation of MPK1/2, and cold tolerance. ABA is also critical for CPK27-induced cold tolerance, generation of H2O2 and NO, and the activation of MPK1/2. These results strongly suggest that CPK27 may function as a positive regulator of ABA generation by activating the production of ROS and NO as well as MPK1/2 in cold adaptation.
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影响因子：
14.9
作者：
Kim SH;Kim HS;Bahk S;An J;Yoo Y;Kim JY;Chung WS
通讯作者：
Chung WS
ZmCPK11 参与脱落酸诱导的抗氧化防御，并在玉米脱落酸信号传导中发挥 ZmMPK5 上游的作用
影响因子：
6.9
作者：
Ding Y;Cao J;Ni L;Zhu Y;Zhang A;Tan M;Jiang M
通讯作者：
Jiang M
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
钙依赖性蛋白激酶 CPK28 以蛋氨酸腺苷转移酶为目标，被 26S 蛋白酶体降解，并影响拟南芥中的乙烯生物合成和木质素沉积
影响因子：
7.2
作者：
Jin, Yu;Ye, Nenghui;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
7.3
作者：
Barrero-Gil, Javier;Huertas, Raul;Salinas, Julio
通讯作者：
Salinas, Julio