Multifunctional superparamagnetic iron oxide nanoparticles for combined chemotherapy and hyperthermia cancer treatment.
Multifunctional superparamagnetic iron oxide nanoparticles for combined chemotherapy and hyperthermia cancer treatment.
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DOI：
10.1039/c5nr02718g
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发表时间：
2015-08-07
期刊：
影响因子：
6.7
通讯作者：
Bao G
中科院分区：
文献类型：
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作者：
Quinto CA;Mohindra P;Tong S;Bao G
超顺磁性氧化铁纳米颗粒（SPIO）具有用作多模式癌症治疗剂的潜力，因为它们能够携带抗癌药物并在暴露于交变磁场时产生局部热，从而导致联合化疗和热疗。为了探索这种潜力，我们合成了具有磷脂-聚乙二醇（PEG）涂层的SPIO，并且当PEG长度被优化时，以30.8%w/w的负载容量负载阿霉素（DOX）。我们发现负载DOX的SPIO在72小时内表现出持续的DOX释放，其中释放动力学可以通过PEG长度改变。相比之下，SPIO的加热效率显示出随PEG长度的最小变化。核心尺寸为14 nm的SPIO可以产生足够的热量，将局部温度升高到43°C，足以引发癌细胞凋亡。此外，我们发现DOX负载的SPIO导致与游离DOX相当的细胞死亡，并且DOX和SPIO诱导的高温的组合作用在体外增强癌细胞死亡。本研究证明了使用磷脂-PEG包被的SPIO用于具有增加的功效的化学疗法-热疗组合癌症治疗的潜力。
Superparamagnetic iron oxide nanoparticles (SPIOs) have the potential for use as a multimodal cancer therapy agent due to their ability to carry anticancer drugs and generate localized heat when exposed to an alternating magnetic field, resulting in combined chemotherapy and hyperthermia. To explore this potential, we synthesized SPIOs with a phospholipid-polyethylene glycol (PEG) coating, and loaded Doxorubicin (DOX) with 30.8% w/w loading capacity when the PEG length is optimized. We found that DOX-loaded SPIOs exhibited a sustained DOX release over 72 hours where the release kinetics could be altered by PEG length. In contrast, the heating efficiency of the SPIOs showed minimal change with PEG length. With a core size of 14 nm, the SPIOs could generate sufficient heat to raise the local temperature to 43°C, enough to trigger apoptosis in cancer cells. Further, we found that DOX-loaded SPIOs resulted in cell death comparable to free DOX, and that the combined effect of DOX and SPIO-induced hyperthermia enhanced cancer cell death in vitro. This study demonstrates the potential of using phospholipid-PEG coated SPIOs for chemotherapy-hyperthermia combinatorial cancer treatment with increased efficacy.