Sequence analysis of the medium (M) segment of Cache Valley virus, with comparison to other Bunyaviridae
Sequence analysis of the medium (M) segment of Cache Valley virus, with comparison to other Bunyaviridae
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DOI：
10.1023/a:1008144808041
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发表时间：
1999-07-01
期刊：
影响因子：
1.6
通讯作者：
Grimstad, PR
中科院分区：
文献类型：
--
作者：
Brockus, CL;Grimstad, PR
利用一系列重叠cDNA克隆，测定了人类神经病原体Cache Valley病毒（CVV）中（M）片段的全序列。病毒互补正义RNA由4463个核苷酸组成，其编码1435个氨基酸的多蛋白前体，起始于碱基49-51的AUG，至碱基4351-4353的UGA终止密码子。该多蛋白编码序列排列为G2-NSm-G1。该片段的碱基组成为34.9% A，17.0% C，19.4% G和28.7% U。与原型Bunyamwera病毒序列的核苷酸序列的比较显示63%的同一性，表明在各个编码区内存在几个差异，最显著的是在NSm和G1编码区内。基于前体内的两个假定的切割点，G2糖蛋白，从nt 94-951编码，是286个氨基酸长，并具有两个潜在的糖基化位点。NSm基因编码的氨基酸序列为nt 952-1476，由175个氨基酸组成，而最大的糖蛋白G1基因编码的氨基酸序列为nt 1477-4350，由958个氨基酸组成，有5个潜在的糖基化位点，其中2个位点是CVV所特有的。这些糖基化位点的后续研究和该原型CVV毒株与其他地理分离株的序列之间的潜在差异可能提示用于改善人类感染的检测以及绘制神经毒力、神经侵袭性和致病性的其他方面的差异的方法。
The complete sequence of the medium (M) segment of Cache Valley virus (CVV), a human neuropathogen, has been determined using a series of overlapping cDNA clones. The viral complementary-sense RNA is comprised of 4463 nucleotides which encodes a polyprotein precursor of 1435 amino acids, starting at AUG at bases 49-51 to a UGA stop codon at bases 4351-4353. This polyprotein-encoding sequence is arranged as G2-NSm-G1. The base composition of the segment is 34.9% A, 17.0% C, 19.4% G and 28.7% U. Comparison of the nucleotide sequence to the prototype Bunyamwera virus sequence shows an identity of 63%, indicating several differences exist within the individual coding regions, most notably within the NSm and G1 coding regions. Based on two presumed cleavage points within the precursor, the G2 glycoprotein, encoded from nt 94-951, is 286 amino acids long, and has two sites of potential glycosylation. NSm, encoded from nt 952-1476, is 175 amino acids, while the largest glycoprotein, G1, encoded from nt 1477-4350, consists of 958 amino acids, and has five potential glycosylation sites, two of which appear to be unique to CVV. The subsequent study of these glycosylation sites and potential differences between the sequence of this prototype CVV strain and other geographic isolates may suggest the means for improving detection of human infections as well as mapping differences in neurovirulence, neuroinvasiveness and other aspects of pathogenicity.