Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts
Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts
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DOI：
10.1007/s11418-018-1223-9
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发表时间：
2018-09-01
影响因子：
3.3
通讯作者：
Shibano, Makio
中科院分区：
文献类型：
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作者：
Kitahiro, Yumi;Koike, Atsushi;Shibano, Makio
麦冬（Opalgon root），其具有滋阴作用，在传统的中国和日本（汉方）医学中，已在临床实践中用于促进液体分泌和滋润肺部和皮肤。为了评价这一传统药物的作用，我们研究了丹参对衰老细胞的抗慢性炎症作用。相反，尽管麦冬（百合科）的几种表型在日本和中国很普遍，但我们将这些麦冬根视为一种生药，即麦冬。本研究发现，原植物O. - 是的从A型中分离出甲基麦冬酮A（化合物1）和甲基麦冬酮B（化合物2）作为指标化合物，从B型中分离出化合物1和麦冬酮A（化合物3）。此外，从A型和B型中分离出麦冬皂苷B（化合物4）作为主要的甾体皂苷。结果表明，A型和B型的两种不同甲醇提取物及O.白细胞介素（IL）-6和IL-8的表达被衰老的正常人皮肤成纤维细胞增强。此外，通过ELISA方法，两种不同的甲醇提取物和化合物1-4以强烈的和浓度依赖性的方式降低IL-6的产生。
Ophiopogonis Radix (Ophiopogon root), which nourishes the yin, has been used in clinical practice to promote fluid secretion and to moisturize the lungs and skin in traditional Chinese and Japanese (Kampo) medicine. To evaluate this traditional medicinal effect, we investigated the anti-chronic inflammatory effect of Radix Ophiopogonis on senescent cells. Conversely, although several phenotypes of Ophiopogon japonicus Ker-Gawler (Liliaceae) are prevalent in Japan and China, we used these Ophiopogon roots by considering them as one crude drug, Ophiopogonis Radix. In this study, it was revealed that there are two chemotypes (Types A and B) in the root of the original plant, O. japonicus. Methylophiopogonanone A (compound 1) and methylophiopogonanone B (compound 2) were isolated as index compounds from Type A and compound 1 and ophiopogonanone A (compound 3) from Type B. In addition, ophiopogonin B (compound 4) was isolated as the main steroidal saponin from both Type A and B. The results indicated that two different methanol extracts (from Types A and B) and the main constituents of O. japonicus (compound 1-4), significantly downregulated the expression of interleukin (IL)-6 and IL-8, which were enhanced by senescent normal human dermal fibroblasts. Moreover, the two different methanol extracts and compounds 1-4 decreased IL-6 production in a strong and concentration-dependent manner by the ELISA method.