The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro
The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro
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DOI：
10.1634/stemcells.2006-0662
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发表时间：
2007-08-01
期刊：
影响因子：
5.2
通讯作者：
Umezawa, Akihiro
中科院分区：
文献类型：
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作者：
Nishiyama, Nobuhiro;Miyoshi, Shunichiro;Umezawa, Akihiro
我们检测了人脐带血来源的间充质干细胞（UCBM-SCs）的心肌样潜能。在有严重冠心病危险因素的老年患者中，干细胞的数量和功能可能受到抑制。因此，从这些患者中获得的干细胞可能不能很好地发挥作用。由于这个原因，UCBMSCs是干细胞治疗的潜在新细胞来源，因为这样的细胞可以从年轻人群中获得，并被常规用于临床患者。将人UCBMSC（5 × 103/cm（2））与胎鼠心肌细胞（[CM] 1 × 105/cm（2））共培养。在共培养的第5天，大约一半的绿色荧光蛋白（GFP）标记的UCBMSCs有节奏地和同步地收缩，表明UCBMSCs之间存在电通信。收缩的UCBMSC的缩短分数为6.5% +/- 0.7%（n = 20）。免疫细胞化学染色显示心肌肌钙蛋白I（清晰条纹+）和连接蛋白43（细胞边缘弥漫性点状染色）阳性。心肌肌钙蛋白-I阳性心肌细胞占GFP标记的UCBM-SC的45% +/-3%。用玻璃微电极记录GFP标记的UCBMSCs的心肌细胞特异性长动作电位时程（186 - 12毫秒）。在与用胶原膜分离的小鼠心肌细胞共培养的UCBMSCs中观察到CM。因此，细胞融合不是UCBMSCs中CM的主要原因。大约一半的人UCBMSCs在体外成功转分化为心肌细胞。脐血间充质干细胞有望成为心脏干细胞治疗的重要细胞来源。
We tested the cardiomyogenic potential of the human umbilical cord blood-derived mesenchymal stem cells (UCBM-SCs). Both the number and function of stem cells may be depressed in senile patients with severe coronary risk factors. Therefore, stem cells obtained from such patients may not function well. For this reason, UCBMSCs are potentially a new cell source for stem cell-based therapy, since such cells can be obtained from younger populations and are being routinely utilized for clinical patients. The human UCBMSCs (5 X 103 per cm(2)) were cocultured with fetal murine cardiomyocytes ([CM] 1 X 10(5) per cm(2)). On day 5 of coeultivation, approximately half of the green fluorescent protein (GFP)-labeled UCBMSCs contracted rhythmically and synchronously, suggesting the presence of electrical communication between the UCBMSCs. The fractional shortening of the contracted UCBMSCs was 6.5% +/- 0.7% (n = 20). The UCBMSC-derived cardiomyocytes stained positive for cardiac troponin-I (clear striation +) and connexin 43 (diffuse dot-like staining at the margin of the cell) by the immunocytochemical method. Cardiac troponin-I positive cardiomyocytes accounted for 45% +/- 3% of GFP-labeled UCBM-SCs. The cardiomyocyte-specific long action potential duration (186 12 milliseconds) was recorded with a glass microelectrode from the GFP-labeled UCBMSCs. CM were observed in UCBMSCs, which were cocultivated in the same dish with mouse cardiomyocytes separated by a collagen membrane. Cell fusion, therefore, was not a major cause of CM in the UCBMSCs. Approximately half of the human UCBMSCs were successfully transdifferentiated into cardiomyocytes in vitro. UCBMSCs can be a promising cellular source for cardiac stem cell-based therapy.