PCDH10 is required for the tumorigenicity of glioblastoma cells
PCDH10 is required for the tumorigenicity of glioblastoma cells
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DOI：
10.1016/j.bbrc.2013.12.138
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发表时间：
2014-01-31
影响因子：
3.1
通讯作者：
Akiyama, Tetsu
中科院分区：
文献类型：
--
作者：
Echizen, Kanae;Nakada, Mitsutoshi;Akiyama, Tetsu
Protocadherin 10（PCDH 10）/OL-protocadherin是一种钙粘蛋白相关的跨膜蛋白，在脑中具有多种作用，包括促进特定的细胞-细胞连接、细胞迁移和轴突引导。最近有报道称，PCDH 10作为肿瘤抑制因子发挥作用，并且其过表达抑制多种肿瘤细胞的增殖或侵袭。然而，PCDH 10在胶质母细胞瘤细胞中的功能尚未阐明。与以前对其他肿瘤的报道相反，我们在这里表明，通过RNA干扰（RNAi）抑制PCDH 10的表达诱导体外胶质母细胞瘤细胞的生长停滞和凋亡。此外，我们证明，敲低PCDH 10抑制胶质母细胞瘤细胞异种移植到免疫功能低下的小鼠的生长。这些结果表明，PCDH 10是胶质母细胞瘤细胞的增殖和致瘤性所必需的。我们推测PCDH 10可能是胶质母细胞瘤治疗的一个有希望的靶点。(C)2014爱思唯尔公司All rights reserved.
Protocadherin10 (PCDH10)/OL-protocadherin is a cadherin-related transmembrane protein that has multiple roles in the brain, including facilitating specific cell-cell connections, cell migration and axon guidance. It has recently been reported that PCDH10 functions as a tumor suppressor and that its overexpression inhibits proliferation or invasion of multiple tumor cells. However, the function of PCDH10 in glioblastoma cells has not been elucidated. In contrast to previous reports on other tumors, we show here that suppression of the expression of PCDH10 by RNA interference (RNAi) induces the growth arrest and apoptosis of glioblastoma cells in vitro. Furthermore, we demonstrate that knockdown of PCDH10 inhibits the growth of glioblastoma cells xenografted into immunocompromised mice. These results suggest that PCDH10 is required for the proliferation and tumorigenicity of glioblastoma cells. We speculate that PCDH10 may be a promising target for the therapy of glioblastoma. (C) 2014 Elsevier Inc. All rights reserved.