A cotton WAKL protein interacted with a DnaJ protein and was involved in defense against Verticillium dahliae
A cotton WAKL protein interacted with a DnaJ protein and was involved in defense against Verticillium dahliae
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棉花 WAKL 蛋白与 DnaJ 蛋白相互作用，参与防御大丽黄萎病
DOI：
10.1016/j.ijbiomac.2020.11.191
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发表时间：
2021-01-15
影响因子：
8.2
通讯作者：
Zhu, Heqin
中科院分区：
文献类型：
--
作者：
Feng, Hongjie;Li, Cheng;Zhu, Heqin
越来越多的证据表明，植物细胞壁相关受体样激酶（WAKs）参与防御病原体的攻击，但其相关的信号传导过程和调控机制仍不清楚。我们从陆地棉（棉）中鉴定了一个wak样激酶（GhWAKL），并对其功能机制进行了表征。GhWAKL在棉花中的表达受大丽花黄萎病诱导，并对水杨酸（SA）的处理有响应。敲低GhWAKL表达可降低SA含量，抑制SA介导的防御反应，增强棉花对大丽花的易感性。在拟南芥中，GhWAKL的生态过表达赋予了植物对病原菌的抗性。进一步分析表明，GhWAKL与细胞膜上的棉花DnaJ蛋白（GhDNAJ1）相互作用。沉默GhDNAJ1也能增强棉花对大丽花病菌的敏感性。此外，GhWAKL在Ser628位点的磷酸化突变降低了GhDNAJ1与植物的相互作用，降低了植物对大百合的抗性。我们认为GhWAKL是提高棉花黄萎病抗性的潜在分子靶点。(C) 2020 Elsevier B.V.版权所有
Accumulating evidence indicates that plant cell wall-associated receptor-like kinases (WAKs) involve in defense against pathogen attack, but their related signaling processes and regulatory mechanism remain largely unknown. We identified a WAK-like kinase (GhWAKL) from upland cotton (Gossypium hirsutum) and characterized its functional mechanism. Expression of GhWAKL in cotton plants was induced by Verticillium dahliae infection and responded to the application of salicylic acid (SA). Knockdown of GhWAKL expression results in the reduction of SA content and suppresses the SA-mediated defense response, enhancing cotton plants susceptibility to V. dahliae. And, ecotopic overexpression of GhWAKL in Arabidopsis thaliana conferred plant resistance to the pathogen. Further analysis demonstrated that GhWAKL interacted with a cotton DnaJ protein (GhDNAJ1) on the cell membrane. Silencing GhDNAJ1 also enhanced cotton susceptibility to V. dahliae. Moreover, the mutation of GhWAKL at site Ser628 with the phosphorylation decreased the interaction with GhDNAJ1 and compromised the plant resistance to V. dahliae. We propose that GhWAKL is a potential molecular target for improving resistance to Verticillium wilt in cotton. (C) 2020 Elsevier B.V. All rights reserved.