Toll-like receptor 4 agonist, lipopolysaccharide, increases the expression levels of cytokines and chemokines in human peripheral blood mononuclear cells.
Toll-like receptor 4 agonist, lipopolysaccharide, increases the expression levels of cytokines and chemokines in human peripheral blood mononuclear cells.
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DOI：
10.3892/etm.2014.2025
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发表时间：
2014-12
影响因子：
2.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Pu D;Wang W
Toll样受体（TLR）是模式识别受体家族的成员，并且在先天免疫应答中是必需的。在人类中总共存在11种TLR，它们在多种细胞中表达，包括外周血细胞。TLR 4通过识别这些细菌中的脂多糖（LPS）分子在抵抗革兰氏阴性病原体中起重要作用。本研究的目的是检测LPS刺激后外周血单个核细胞（PBMC）中一些主要免疫分子的表达水平变化，以确定参与TLR 4介导的生物学功能的候选基因。在本研究中进行了逆转录定量聚合酶链反应（RT-qPCR）分析和抗体芯片。RT-qPCR结果显示各种分子的表达水平显著增强，包括细胞因子、趋化因子、生长因子和蛋白激酶。此外，抗体芯片鉴定了从LPS处理的PBMC收集的上清液中关键促炎分子的分泌增加。总之，发现大量分子参与TLR 4介导的功能。
Toll-like receptors (TLRs) are members of the pattern recognition receptor family and are essential in the innate immune response. In total, 11 TLRs exist in humans, which are expressed in a variety of cells, including peripheral blood cells. TLR4 plays a significant role in the defense against gram-negative pathogens by recognizing the lipopolysaccharide (LPS) molecules in these bacteria. The aim of the present study was to detect the expression level variation of a number of major immune molecules in peripheral blood mononuclear cells (PBMCs) stimulated by LPS, in order to identify candidate genes involved in the biological functions mediated by TLR4. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis and an antibody chip were performed in the current study. The RT-qPCR results revealed a marked enhancement in the expression levels of various molecules, including cytokines, chemokines, growth factors and protein kinases. In addition, the antibody chip identified the increased secretion of crucial proinflammatory molecules in the supernatants collected from LPS-treated PBMCs. In conclusion, a large number of molecules were found to be involved in TLR4-mediated functions.
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