FIBROBLAST GROWTH-FACTOR IN THE HYPOTHALAMIC-PITUITARY AXIS - DIFFERENTIAL EXPRESSION OF FIBROBLAST GROWTH FACTOR-II AND A HIGH-AFFINITY RECEPTOR
FIBROBLAST GROWTH-FACTOR IN THE HYPOTHALAMIC-PITUITARY AXIS - DIFFERENTIAL EXPRESSION OF FIBROBLAST GROWTH FACTOR-II AND A HIGH-AFFINITY RECEPTOR
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DOI：
10.1210/en.134.5.2289
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发表时间：
1994-05-01
期刊：
影响因子：
4.8
通讯作者：
BAIRD, A
中科院分区：
文献类型：
--
作者：
GONZALEZ, AM;LOGAN, A;BAIRD, A
应用原位杂交和免疫组织化学技术研究了碱性成纤维细胞生长因子2（FGF-2）在下丘脑-垂体系统的基因表达和蛋白分布。虽然FGF-2 mRNA在垂体中的表达较低，但其蛋白质广泛分布于其神经叶和前叶。在前叶，免疫反应性（ir-）FGF-2定位于基底膜和选择内分泌细胞。在神经叶中，在基底膜、垂体细胞和Herring小体中检测到ir-FGF-2。对垂体前叶中FGF高亲和力受体（FGFR）免疫反应性的分析表明，FGFR的分布与FGF-2相似。在神经叶中，ir-FGFR与神经纤维、垂体细胞和Herring小体相关。与FGF-2不同，FGFR 1 mRNA的分布与免疫反应性受体的存在密切相关。在下丘脑，室旁核和视上核的大细胞神经元含有ir-FGF-2和ir-FGFR。在正中隆起中，ir-FGF-2和ir-FGFR与纤维、神经胶质和内皮细胞相关。衬于第三脑室的室管膜和室管膜下细胞也显示高水平的ir-FGF-2和ir-FGFR和mRNAs。总体而言，FGF-2及其高亲和力受体在下丘脑-垂体轴中存在特异性和选择性分布。这种定位导致我们假设在神经垂体功能中的作用，可能是水平衡。
In situ hybridization and immunohistochemistry were used to map gene expression and protein distribution of basic fibroblast growth factor (FGF-2) in the hypothalamic-pituitary system. Although the expression of FGF-2 mRNA in the pituitary is low, the protein is widely distributed in both its neural and anterior lobes. In the anterior lobe, immunoreactive (ir-) FGF-2 localizes to basement membranes and select endocrine cells. In the neural lobe, ir-FGF-2 is detected in basement membranes, pituicytes, and Herring bodies. Analyses of FGF high affinity receptor (FGFR) immunoreactivity in the anterior pituitary establishes a distribution of FGFR similar to that of FGF-2. In the neural lobe, ir-FGFR is associated with nerve fibers, pituicytes, and Herring bodies. Unlike FGF-2, the distribution of FGFR1 mRNA correlates well with the presence of the immunoreactive receptor. In the hypothalamus, magnocellular neurons of paraventricular and supraoptic nuclei contain ir-FGF-2 and ir-FGFR. In the median eminence, ir-FGF-2 and ir-FGFR is associated with fibers, glial, and endothelial cells. Ependymal and subependymal cells lining the third ventricle also show high levels of ir-FGF-2 and ir-FGFR and mRNAs.Overall, there is a specific and selective distribution of FGF-2 and its high affinity receptor(s) in the hypothalamo-pituitary axis. This localization lead us to postulate a role in neurohypophyseal functions, possibly water balance.