SPECIFIC CYTOCHEMICAL DEMONSTRATION IN ELECTRON MICROSCOPE OF PERIODATE-REACTIVE MUCOSUBSTANCES AND POLYSACCHARIDES CONTAINING VIC-GLYCOL GROUPS
SPECIFIC CYTOCHEMICAL DEMONSTRATION IN ELECTRON MICROSCOPE OF PERIODATE-REACTIVE MUCOSUBSTANCES AND POLYSACCHARIDES CONTAINING VIC-GLYCOL GROUPS
复制标题
DOI：
10.1007/bf00326613
复制
发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
STOWARD, PJ
中科院分区：
文献类型：
--
作者：
BRADBURY, S;STOWARD, PJ
介绍了一种在电子显微镜下定位高碘酸盐反应性粘液基质和含有Vic-乙二醇基的多糖的技术。在这种技术中，糖残留物被高碘酸氧化，生成的醛在特定条件下与五氟苯肼缩合。进一步提高比电子密度的方法是用硫化铵处理最终产物，然后用四氧化二臭氧处理，以形成臭氧黑。这项技术被应用于肝和小肠细胞[大鼠]，其中糖原、唾液粘蛋白和宿命的粘膜物质反应特别强烈。间质细胞基质、高尔基体和肠上皮细胞的多泡小体也呈明显阳性反应。反应可以通过释放高碘酸氧化来防止，如果是由于糖原，可以通过预先用淀粉酶处理来防止反应。
A technique is described for the localization in the electron microscope of periodate-reactive mucosubstances and polysaccharides containing vic-glycol groups. In this technique the sugar residues are oxidized by periodic acid and the resulting aldehydes condensed with pentafluorophenylhydrazine under specified conditions. Further increase in specific electron density is achieved by treating the hydrazone end-product with ammonium sulfide followed by osmium tetraxide to form an osmium black. The technique was applied to liver and small intestine cells [rat] in which glycogen, sialomucins and sul-fated mucosubstances reacted especially strongly. A marked positive reaction was also obtained from the interstitial cell matrix and from the Golgi apparatus and multivesicular bodies of the intestinal epithelial cells. The reaction can be prevented by the emission of the periodate oxidation and, if due to glycogen, by prior treatment with diastase.