MECHANISM OF OZONE INACTIVATION OF BACTERIOPHAGE-F2
MECHANISM OF OZONE INACTIVATION OF BACTERIOPHAGE-F2
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DOI：
10.1128/aem.39.1.210-218.1980
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发表时间：
1980-01-01
影响因子：
4.4
通讯作者：
SPROUL, OJ
中科院分区：
文献类型：
--
作者：
KIM, CK;GENTILE, DM;SPROUL, OJ
在实验室控制条件下，研究了O_3对噬菌体f_2的灭活动力学。噬菌体迅速灭活在第一个5秒的反应由5和7个日志在O3浓度为0.09和0.8毫克/升，分别。在接下来的10分钟期间，在两种处理中以较慢的速率进一步灭活噬菌体。[3 H]尿苷标记的f2噬菌体和它的RNA进行了检查，以阐明O3灭活的机制，利用吸附宿主细菌[大肠杆菌]，蔗糖密度梯度分析和EM。臭氧氧化降低了噬菌体的特异性吸附，其模式与噬菌斑形成单位的减少相同。RNA从噬菌体颗粒在臭氧氧化过程中释放，虽然它降低了原生质球的感染性。电镜观察表明，臭氧氧化可使噬菌体外壳破碎成许多蛋白质亚基碎片，噬菌体对宿主皮利的特异性吸附与噬菌体破碎程度呈负相关。封闭在噬菌体外壳中的RNA被臭氧灭活的程度低于整个噬菌体，但比裸RNA灭活的程度更高。显然，O3将蛋白质衣壳破坏成亚单位，释放RNA并破坏对宿主皮利的吸附，RNA可能通过有和/或没有被臭氧化修饰的外壳蛋白分子的还原而被二次剪切。
The inactivation kinetics of bacteriophage f2 were studied by using O3 under controlled laboratory conditions. The phage were rapidly inactivated during the first 5 s of the reaction by 5 and 7 logs at O3 concentrations of 0.09 and 0.8 mg/l, respectively. During the next 10 min, the phage were further inactivated at a slower rate in both treatments. [3H]uridine-labeled f2 phage and its RNA were examined to elucidate the mechanism of O3 inactivation, utilizing adsorption to host bacteria [Escherichia coli], sucrose density gradient analysis and EM. The specific adsorption of the phage was reduced by ozonation in the same pattern as plaque-forming unit reduction. RNA was released from the phage particles during ozonation, although it had reduced infectivity for spheroplasts. EM examination showed that the phage coat was broken by ozonation into many protein subunit pieces and that the specific adsorption of the phage to host pili was inversely related to the extent of phage breakage. The RNA enclosed in the phage coat was inactivated less by ozonation than were whole phage, but inactivated more than naked RNA. Apparently O3 breaks the protein capsid into subunits, liberating RNA and disrupting adsorption to the host pili, the RNA may be secondarily sheared by a reduction with and/or without the coat protein molecules which were modified by ozonation.