Prolonged Cold Ischemia Time in Mouse Heart Transplantation Using Supercooling Preservation
Prolonged Cold Ischemia Time in Mouse Heart Transplantation Using Supercooling Preservation
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DOI：
10.1097/tp.0000000000003089
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发表时间：
2019-12
期刊：
影响因子：
6.2
通讯作者：
Weitao Que;Xin Hu;M. Fujino;H. Terayama;K. Sakabe;N. Fukunishi;P. Zhu;Shuang-Qin Yi;Y. Yamada;Lin Zhong;Xiao‐Kang Li
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文献类型：
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作者：
Weitao Que;Xin Hu;M. Fujino;H. Terayama;K. Sakabe;N. Fukunishi;P. Zhu;Shuang-Qin Yi;Y. Yamada;Lin Zhong;Xiao‐Kang Li
补充数字内容可在文本中找到。背景过冷保存技术将供体器官储存在0°C以下而不冷冻。与4°C的常规保存相比，这在抑制代谢和保存器官方面具有很大的优势。我们开发了一种新的过冷技术，使用液体冷却装置和新的保存和灌注解决方案。本研究的目的是评价我们的过冷保存技术在小鼠心脏移植模型中的保存效果。方法.将同基因小鼠异位心脏移植分为3组：（1）非保存组，取心后立即移植;（2）常规威斯康星州（UW）组，取心后置于UW液中4°C保存不同时间;（3）过冷组，采用一种新型的过冷保存液，在-8 ℃下保存不同时间。考察了最长保存时间。收集并分析了24小时的样品数据，以比较过冷保存和传统的UW保存。结果我们的技术产生了稳定的-8 ° C过冷状态。过冷保存144 h后移植心脏复苏成功，过冷保存96 h后移植心脏长期存活。与传统的4°C UW保存相比，移植后结果，包括心肌缺血-再灌注损伤、氧化应激相关损伤和心肌细胞凋亡得到改善。结论.与传统的4°C UW保存相比，在-8 ° C下过冷保存心脏大大延长了保存时间并改善了移植后结果。过冷保存是一种很有前途的器官保存技术。
Supplemental Digital Content is available in the text. Background. Supercooling preservation techniques store a donor organ below 0°C without freezing. This has great advantages in inhibiting metabolism and preserving the organ in comparison to conventional preservation at 4°C. We developed a novel supercooling technique using a liquid cooling apparatus and novel preservation and perfusion solutions. The purpose of this study was to evaluate the preservation effect of our supercooling preservation technique in a mouse heart transplantation model. Methods. Syngeneic heterotopic heart transplantation was performed in 3 groups of mice: (1) the nonpreservation group, in which the cardiac grafts were transplanted immediately after retrieval; (2) the conventional University of Wisconsin (UW) group, in which the cardiac grafts were stored in UW solution at 4°C for different periods of time; and (3) the supercooling group, in which the cardiac grafts were stored in a novel supercooling preservation solution at –8°C for different periods of time. The maximal preservation time was investigated. Twenty-four-hour sample data were collected and analyzed to compare supercooling preservation to conventional UW preservation. Results. Our technique yielded a stable –8°C supercooling state. Cardiac graft revival was successfully achieved after supercooling preservation for 144 hours, and long-term survival was observed after supercooling preservation for 96 hours. Posttransplant outcomes, including myocardial ischemia–reperfusion injury, oxidative stress-related damage, and myocardial cell apoptosis, were improved in comparison to conventional 4°C UW preservation. Conclusions. Supercooling heart preservation at –8°C greatly prolonged the preservation time and improved the posttransplant outcomes in comparison to conventional 4°C UW preservation. Supercooling preservation is a promising technique for organ preservation.