Expression of stilbene synthase VqSTS6 from wild Chinese Vitis quinquangularis in grapevine enhances resveratrol production and powdery mildew resistance
Expression of stilbene synthase VqSTS6 from wild Chinese Vitis quinquangularis in grapevine enhances resveratrol production and powdery mildew resistance
复制标题
野生五角葡萄的二苯乙烯合酶 VqSTS6 在葡萄中的表达可增强白藜芦醇的产量和白粉病抗性
DOI：
10.1007/s00425-019-03276-2
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发表时间：
2019-12-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Yuejin
中科院分区：
文献类型：
--
作者：
Liu, Mengqi;Ma, Fuli;Wang, Yuejin
在葡萄（葡萄属）中，二苯乙烯植物抗毒素可以在白粉病感染过程中原位合成或运输到反应部位，增强抗病性。白藜芦醇是一种植物保护性芪类化合物，由芪合酶（STS）合成，以应对生物和非生物胁迫，也被称为在人类饮食中具有健康益处。我们以前已经表明，转基因葡萄属vinifera cv。过量表达中国野生葡萄属葡萄二苯乙烯合成酶基因VqSTS 6的Thompson无核植株具有较高的二苯乙烯类化合物含量，从而增强了对白粉病（Uncinula necator（Schw.）毛刺）。然而，在白粉病感染下，植物组织中的生物合成和运输仍不清楚。本文采用抑制剂和微嫁接技术研究了白藜芦醇在白粉病侵染下的积累。我们观察到，STS的表达和芪类化合物的水平增加响应白粉病感染。葡萄离体枝条经白粉病和抑制剂处理后，白藜芦醇是原位合成的。嫁接植株的实验表明，在根中过表达VqSTS 6期间，茎中芪类化合物的丰度增加，而VqSTS 6-Flag融合体不转运到接穗，仅在转基因砧木中表达。与野生型Thompson无核植株相比，非转基因/VqSTS 6转基因（接穗/砧木）嫁接Thompson无核植株表现出对白粉病的抗性增强。此外，VqSTS 6在根中的过表达导致其他五个欧洲葡萄品种（V. vinifera cvs.霞多丽，佩莱特，赤霞珠，雷司令和麝香汉堡）。总之，芪类化合物可以在原位合成或运输到白粉病感染的网站，和VqSTS 6在根中的过表达促进芪类化合物的积累和抗病性在欧洲葡萄藤品种。
In grape (Vitis), stilbene phytoalexins can either be in situ synthesized or transported to the site of response during powdery mildew infection, enhancing disease resistance. Resveratrol is a phytoprotective stilbenoid compound that is synthesized by stilbene synthase (STS) in response to biotic and abiotic stresses, and is also known to have health benefits in the human diet. We have previously shown that transgenic Vitis vinifera cv. Thompson Seedless plants overexpressing a stilbene synthase gene, VqSTS6, from wild Chinese Vitis quinquangularis had a higher stilbenoid content, leading to an enhanced resistance to powdery mildew (Uncinula necator (Schw.) Burr). However, the biosynthesis and transportation in the plant tissue under powdery mildew infection are still unclear. Here, inhibitor and micro-grafting technologies were used to study the accumulation of resveratrol following powdery mildew infection. We observed that the levels of STS expression and stilbenoids increased in response to powdery mildew infection. Powdery mildew and inhibitor treatment on detached grape branches showed that resveratrol was in situ synthesized. Experiments with grafted plantlets showed that the abundance of stilbenoid compounds increased in the shoot during VqSTS6 overexpression in the root, while VqSTS6-Flag fusion was not tranported to the scions and only expressed in the transgenic rootstocks. Compared with wild-type Thompson Seedless plants, the non-transgenic/VqSTS6 transgenic (scion/rootstock) grafted Thompson Seedless plantlets exhibited increased resistance to powdery mildew. In addition, overexpression of VqSTS6 in roots led to increased levels of stilbenoid compounds in five other European grape varieties (V. vinifera cvs. Chardonnay, Perlette, Cabernet Sauvignon, Riesling and Muscat Hamburg). In conclusion, stilbenoid compounds can be either in situ synthesized or transported to the site of powdery mildew infection, and overexpression of VqSTS6 in the root promotes stilbenoids accumulation and disease resistance in European grapevine varieties.