SCY1-like 1 binding protein 1 (SCYL1-bp1) interacts with p53-induced RING H2 protein (Pirh2) after traumatic brain injury in rats
SCY1-like 1 binding protein 1 (SCYL1-bp1) interacts with p53-induced RING H2 protein (Pirh2) after traumatic brain injury in rats
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DOI：
10.1007/s10735-013-9488-5
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发表时间：
2013-06-01
影响因子：
3.2
通讯作者：
Shi, Wei
中科院分区：
文献类型：
--
作者：
Chen, Jian;Zou, Feihui;Shi, Wei
创伤性脑损伤会引发一系列复杂的神经化学和信号变化，导致神经元功能障碍和星形胶质细胞过度反应。在本研究中，我们发现SCYL1-bp1和Pirh2之间的相互作用参与了中枢神经系统(CNS)的损伤和修复。Western印迹和免疫组织化学分析显示，与对侧大脑皮质相比，成年大鼠急性创伤性脑损伤模型同侧大脑皮质中SCYL1-bp1和Pirh2的水平显著增加。免疫荧光双标分析进一步表明，SCYL1-bp1主要与NeuN共表达。末端脱氧核苷酸转移酶介导的生物素化dUTP缺口末端标记结果支持SCYL1-bp1和Pirh2参与脑损伤后神经元的凋亡。此外，我们还检测了活性caspase-3的表达谱，这些表达谱与SCYL1-bp1和Pirh2的水平相关。值得注意的是，SCYL1-bp1和Pirh2都与激活的caspase-3共定位，这三种蛋白都参与了神经元的凋亡。免疫沉淀实验进一步揭示了这些蛋白质在病理生理过程中的相互作用。据我们所知，这是第一个报道SCYL1-bp1和Pirh2在创伤性脑中相互作用的研究。我们的研究结果表明SCYL1-bp1和Pirh2在脑外伤后中枢神经系统的病理生理学中起重要作用。
Traumatic brain injury (TBI) triggers a complex series of neurochemical and signaling changes that lead to neuronal dysfunction and overreactive astrocytes. In the current study, we showed that interactions between SCYL1-bp1 and Pirh2 are involved in central nervous system (CNS) injury and repair. Western blot and immunohistochemical analysis of an acute traumatic brain injury model in adult rats revealed significantly increased levels of SCYL1-bp1 and Pirh2 in the ipsilateral brain cortex, compared to contralateral cerebral cortex. Immunofluorescence double-labeling analyses further revealed that SCYL1-bp1 is mainly co-expressed with NeuN. Terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling staining data supported the involvement of SCYL1-bp1 and Pirh2 in neuronal apoptosis after brain injury. We additionally examined the expression profiles of active caspase-3, which were altered in correlation with the levels of SCYL1-bp1 and Pirh2. Notably, both SCYL1-bp1 and Pirh2 were colocalized with active caspase-3, and all three proteins participated in neuronal apoptosis. Immunoprecipitation experiments further revealed interactions of these proteins with each other in the pathophysiology process. To our knowledge, this is the first study to report interactions between SCYL1-bp1 and Pirh2 in traumatic brain. Our data collectively indicate that SCYL1-bp1 and Pirh2 play important roles in CNS pathophysiology after TBI.