Quorum-Sensing Control of Antibiotic Synthesis in Burkholderia thailandensis
Quorum-Sensing Control of Antibiotic Synthesis in Burkholderia thailandensis
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DOI：
10.1128/jb.00200-09
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发表时间：
2009-06-15
影响因子：
3.2
通讯作者：
Greenberg, E. Peter
中科院分区：
文献类型：
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作者：
Duerkop, Breck A.;Varga, John;Greenberg, E. Peter
Burkholderia thailandensis的基因组编码几个LuxR-LuxI群体感应系统。我们用了B。thailandensis群体感应缺失突变体和重组大肠杆菌，以确定由系统之一BtaR 2-BtaI 2产生的信号的性质，并显示该系统控制抗生素合成所需的基因。BtaI 2是酰基-高丝氨酸内酯（酰基-HSL）合酶，其产生两种羟基化酰基-HSL，N-3-羟基-癸酰基-HSL（3OHC（10）-HSL）和N-3-羟基-辛酰基-HSL（3OHC（8）-HSL）。BtaI 2基因受BtaR 2的正调控，以响应3OHC（10）-HSL或3OHC（8）-HSL。btaR 2-btaI 2基因位于具有注释的基因簇内，这些注释表明它们参与聚酮化合物或肽抗生素的合成。野生型B的稳定期培养物。thailandensis，而不是btaR 2突变体或酰基-HSL合成缺陷的菌株，产生有效对抗革兰氏阳性细菌的抗生素。两个推定的抗生素合成基因簇需要BtaR 2和3OHC（10）-HSL或3OHC（8）-HSL来激活。这代表了另一个抗生素合成受群体感应控制的例子，它对B的进化分歧有影响。thailandensis及其近缘种类鼻疽伯克霍尔德氏菌和鼻疽伯克霍尔德氏菌。
The genome of Burkholderia thailandensis codes for several LuxR-LuxI quorum-sensing systems. We used B. thailandensis quorum-sensing deletion mutants and recombinant Escherichia coli to determine the nature of the signals produced by one of the systems, BtaR2-BtaI2, and to show that this system controls genes required for the synthesis of an antibiotic. BtaI2 is an acyl-homoserine lactone (acyl-HSL) synthase that produces two hydroxylated acyl-HSLs, N-3-hydroxy-decanoyl-HSL (3OHC(10)-HSL) and N-3-hydroxy-octanoyl-HSL (3OHC(8)-HSL). The btaI2 gene is positively regulated by BtaR2 in response to either 3OHC(10)-HSL or 3OHC(8)-HSL. The btaR2-btaI2 genes are located within clusters of genes with annotations that suggest they are involved in the synthesis of polyketide or peptide antibiotics. Stationary-phase cultures of wild-type B. thailandensis, but not a btaR2 mutant or a strain deficient in acyl-HSL synthesis, produced an antibiotic effective against gram-positive bacteria. Two of the putative antibiotic synthesis gene clusters require BtaR2 and either 3OHC(10)-HSL or 3OHC(8)-HSL for activation. This represents another example where antibiotic synthesis is controlled by quorum sensing, and it has implications for the evolutionary divergence of B. thailandensis and its close relatives Burkholderia pseudomallei and Burkholderia mallei.