The response of human sulcular leucocytes to a chemotactic challenge. A new in vivo assay.
The response of human sulcular leucocytes to a chemotactic challenge. A new in vivo assay.
复制标题
人类龈沟白细胞对趋化挑战的反应。
DOI：
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发表时间：
1981
影响因子：
3.5
通讯作者：
R. Kaslick
中科院分区：
文献类型：
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作者：
L. Golub;V. Iacono;G. Nicoll;N. Ramamurthy;R. Kaslick
我们监测了人类和大鼠的龈沟白细胞（CL）和液体（CF）在体内的化学挑战的反应，试图开发一种体内趋化性测定。将牙科男学生的#21和#22的近中裂隙（平均GI = 0;裂隙深度= 2.3mm）洗涤并风干。记录CL（#21）和CF（#22）的基线值。在10 μ l缝隙冲洗液中收集CL，并立即在血细胞计数器中计数等分试样。在滤纸条上收集CF并用流量计测量。酪蛋白，一种营养物质和一种标准的体外化学引诱剂，用一个校准的金属丝环施加到每个缝隙中。CL和CF监测15分钟后，在5分钟的时间间隔长达45分钟。CL和CF达到峰值约30分钟后，酪蛋白的挑战，这两个参数迅速恢复到基线之后。在大鼠中，当将浓度为0.01-4.0 mg/ml的酪蛋白应用于龈沟时，获得了剂量反应。CL和CF的最大响应发生在2.0 mg/ml。单独应用缓冲液或缺乏趋化活性的Fab蛋白，不产生CL和CF的升高。我们认为，这种裂隙技术可用于监测白细胞趋化性缺陷的受试者的体内反应。
We monitored the in vivo response of human and rat crevicular leucocytes (CL) and fluid (CF) to a chemical challenge in an attempt to develop an in vivo chemotaxis assay. The mesial crevices of #21 and #22 of male dental students (mean GI = 0; crevice depth = 2.3 mm) were washed and air dried. The baseline values for CL (#num;21) and CF (#22) were recorded. CL were collected in 10 μI washes of the crevice and an aliquot immediately counted in a hemacytometer. CF was collected on filter paper strips and measured with a fluid meter. Casein, a nutrient and a standard in vitro chemo-attractant was applied with a calibrated wire loop into each crevice. CL and CF were monitored 15 min later and at 5 min intervals up to 45 min. CL and CF peaked approximately 30 min after the casein challenge; both parameters returned to baseline rapidly thereafter. In rats, a dose-response was obtained when concentrations of 0.01–4.0 mg casein/ml were applied to the gingival crevice. The maximum response of CL and CF occurred at 2.0 mg/ml. The application of buffer alone or Fab protein, which lacks chemotactic activity, produced no rise in CL and CF. We suggest that this crevicular technique can be used to monitor the in vivo response of subjects with defects in leucocyte chemotaxis.