PSEUDOMONAS-AERUGINOSA CYTOTOXIN AS A PATHOGENICITY FACTOR IN A SYSTEMIC INFECTION OF LEUKOPENIC MICE
PSEUDOMONAS-AERUGINOSA CYTOTOXIN AS A PATHOGENICITY FACTOR IN A SYSTEMIC INFECTION OF LEUKOPENIC MICE
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DOI：
10.1016/0041-0101(94)90018-3
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发表时间：
1994-01-01
期刊：
影响因子：
2.8
通讯作者：
LUTZ, F
中科院分区：
文献类型：
--
作者：
BALTCH, AL;SMITH, RP;LUTZ, F
利用一株细胞毒素高产菌株(PA158)及其经转座子Tn7：：Tn5转座子诱变产生的细胞毒素缺陷型等位基因突变株(PA114F5)，对白血球减少症雄性小鼠(20g)进行了铜绿假单胞菌细胞毒素作用的评价。用腹腔注射(Ip)诱发白细胞减少。给予环磷酰胺(150微克/克)。麻醉小鼠以300CFU/只(9LD(50)；预期死亡率85%)经剃背4 mm切口处感染。用预清的小鼠细胞毒素比热灭活兔多克隆抗体(RPA)腹腔注射。(0.2毫升)，激发前24小时。控制收到的IP。正常兔血清，RPA，单独环磷酰胺，或假手术。与细胞毒素缺乏菌株PA114F5相比，高细胞毒素产生菌株PA158攻击的时间更早，死亡率更高(P<；0.01)。细胞毒素特异性多克隆抗体具有保护性作用。抗体预处理使PA158攻击后的死亡率从88.9%降至27.8%(P<；0.01)。抗体预处理使PA114F5攻击后的死亡率从27.8%降至5.6%(P<；0.05)。这些结果表明，铜绿假单胞菌细胞毒素参与了机体的致病性，细胞毒素抗体对白细胞减少症小鼠的全身性铜绿假单胞菌感染具有保护作用。
The effect of Pseudomonas aeruginosa cytotoxin was assessed in leukopenic outbred Swiss male mice (20 g) using a high cytotoxin-producing strain (PA158) and its cytotoxin-deficient isogenic mutant (PA114F5) generated by Tn7::Tn5 transposon mutagenesis of PA 158. Leukopenia was induced by intraperitoneal (i.p.) administration of cyclophosphamide (150 mu g/g). Anesthetized mice were infected via a 4 mm incision on the shaved back with 300 CFU/mouse (9 LD(50); expected death rate 85%). Precleared mouse cytotoxin-specific heat inactivated rabbit polyclonal antibody (RPA) was administered i.p. (0.2 ml) 24 hr before challenge. Controls received i.p. normal rabbit serum, RPA, cyclophosphamide alone, or a sham procedure. Challenge with the high cytotoxin-producing strain PA158 caused earlier and a significantly greater mortality than that observed with a cytotoxin-deficient strain PA114F5 (P < 0.01). Cytotoxin-specific polyclonal antibody was protective. Pretreatment with antibody decreased the mortality rate following challenge with PA158 from 88.9% to 27.8% (P < 0.01). Pretreatment with antibody decreased the mortality rate following challenge with PA114F5 from 27.8% to 5.6% (P < 0.05). These results demonstrate that P. aeruginosa cytotoxin contributes to the pathogenicity of the organism and that cytotoxin antibody is protective in a systemic P. aeruginosa infection in leukopenic mice.