UT-B1 proteins in rat: tissue distribution and regulation by antidiuretic hormone in kidney
UT-B1 proteins in rat: tissue distribution and regulation by antidiuretic hormone in kidney
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DOI：
10.1152/ajprenal.00359.2001
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发表时间：
2002-11-01
影响因子：
4.2
通讯作者：
Bailly, P
中科院分区：
文献类型：
--
作者：
Trinh-Trang-Tan, MM;Lasbennes, F;Bailly, P
UT-B1是肾脏内红细胞（rbc）和直血管降支内皮细胞的尿素转运蛋白。用抗大鼠UT-B1 C-ter序列的多克隆抗体免疫印迹显示，UT-B1在大鼠红细胞膜、肾髓质、脑和膀胱中同时存在非糖基化（29 kDa）和n糖基化（47.5和33 kDa）蛋白。在睾丸中，UT-B1仅以47.5 kDa的非糖基化蛋白表达。免疫细胞化学证实UT-B1的位置仅限于直肠降血管。在脑组织星形胶质细胞和室管膜细胞中发现UT-B1蛋白。星形胶质细胞的细胞体和血管周围端足被标记在大脑皮层，而星形胶质细胞的细胞突被标记在胼胝体。红细胞的流式细胞术分析显示该抗体与小鼠和人UT-B1具有良好的交叉反应性。长期输注抗利尿素[去氨基-胱氨酸(1)，d -精氨酸(8)]可显著下调大鼠肾髓质的UT-B1蛋白表达，速尿治疗可适度下调其表达。本研究揭示了星形胶质细胞内UT-B1蛋白的不均匀分布以及抗利尿激素对肾脏UT-B1蛋白的调节作用。
UT-B1 is the facilitated urea transporter of red blood cells (RBCs) and endothelial cells of descending vasa recta in the kidney. Immunoblotting with a polyclonal antibody against the C-ter sequence of rat UT-B1 revealed UT-B1 as both nonglycosylated (29 kDa) and N-glycosylated (47.5 and 33 kDa) proteins in RBC membranes, kidney medulla, brain, and bladder in rat. In testis, UT-B1 was expressed only as a nonglycosylated protein of 47.5 kDa. Immunocytochemistry confirmed that the location of UT-B1 is restricted to descending vasa recta. In brain, UT-B1 protein was found in astrocytes and ependymal cells. Cell bodies and perivascular end feet of astrocytes were labeled in brain cortex, whereas astrocyte cell processes were labeled in corpus callosum. Flow cytometry analysis of RBCs revealed a good cross-reactivity of the antibody with mouse and human UT-B1. UT-B1 protein expression in rat kidney medulla was downregulated greatly by long-term [deamino-Cys(1),D-Arg(8)]vasopressin infusion and moderately by furosemide treatment. This study discloses an uneven distribution of UT-B1 protein within astrocytes and the regulation of renal UT-B1 protein by antidiuretic hormone.