Temperature changes in the root ecosystem affect plant functionality.
Temperature changes in the root ecosystem affect plant functionality.
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DOI：
10.1016/j.xplc.2022.100514
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发表时间：
2023-05-08
影响因子：
10.5
通讯作者：
del Pozo, Juan C.
中科院分区：
文献类型：
--
作者：
Gonzalez-Garcia, Mary Paz;Conesa, Carlos M.;Lozano-Enguita, Alberto;Baca-Gonzalez, Victoria;Simancas, Barbara;Navarro-Neila, Sara;Sanchez-Bermudez, Maria;Salas-Gonzalez, Isai;Caro, Elena;Castrillo, Gabriel;del Pozo, Juan C.
气候变化正在增加极端高温事件的频率，加剧其对植物发育和农业产量的负面影响。大多数旨在研究植物对热胁迫的适应性的实验对茎和根施加均匀的高温。然而，这种处理不能模拟自然田地中的条件，在自然田地中，根生长在具有下降的温度梯度的黑暗环境中。过高的温度会严重降低根分生组织中的细胞分裂，损害根的生长，同时增加静止中心细胞的分裂，可能是为了在这种恶劣条件下维持干细胞生态位。在这里，我们设计了TGRooZ，这是一种为体外或温室生长测定产生温度梯度的设备。暴露于高芽温度但在TGRooZ中栽培的植物的根系有效地生长并保持其功能以维持适当的芽生长和发育。此外，基因表达和根际或根微生物组组成在TGRooZ生长的根中比在高温生长的根中受影响显著更小，与更高的根功能相关。我们的数据表明，在热胁迫研究中使用TGRooZ可以提高我们对植物对高温反应的认识，证明其从实验室研究到田间的适用性。气候变化和热量是植物生长和生产力的限制因素。这项研究提供了新的见解植物热响应使用一种创新的设备，产生一个温度梯度的根系，模拟自然土壤条件。
Climate change is increasing the frequency of extreme heat events that aggravate its negative impact on plant development and agricultural yield. Most experiments designed to study plant adaption to heat stress apply homogeneous high temperatures to both shoot and root. However, this treatment does not mimic the conditions in natural fields, where roots grow in a dark environment with a descending temperature gradient. Excessively high temperatures severely decrease cell division in the root meristem, compromising root growth, while increasing the division of quiescent center cells, likely in an attempt to maintain the stem cell niche under such harsh conditions. Here, we engineered the TGRooZ, a device that generates a temperature gradient for in vitro or greenhouse growth assays. The root systems of plants exposed to high shoot temperatures but cultivated in the TGRooZ grow efficiently and maintain their functionality to sustain proper shoot growth and development. Furthermore, gene expression and rhizosphere or root microbiome composition are significantly less affected in TGRooZ-grown roots than in high-temperature-grown roots, correlating with higher root functionality. Our data indicate that use of the TGRooZ in heat-stress studies can improve our knowledge of plant response to high temperatures, demonstrating its applicability from laboratory studies to the field. Climate change and heat are limiting factors for plant growth and productivity. This study provides new insight into plant heat response using an innovative device that generates a temperature gradient in the root system, simulating natural soil conditions.
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影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
9.8
作者：
Finkel, Omri M.;Salas-Gonzalez, Isai;Dangl, Jeffery L.
通讯作者：
Dangl, Jeffery L.
影响因子：
11
作者：
Ferrenberg, Scott;O'Neill, Sean P.;Nemergut, Diana R.
通讯作者：
Nemergut, Diana R.
影响因子：
7.4
作者：
Aung, Kyaw;Lin, Shu-I;Chiou, Tzyy-Jen
通讯作者：
Chiou, Tzyy-Jen
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y