Effect of the matrix on DNA electrophoretic mobility.

Effect of the matrix on DNA electrophoretic mobility.
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DOI:
10.1016/j.chroma.2008.11.090
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发表时间:
2009-03-06
期刊:
Journal of chromatography. A
影响因子:
--
通讯作者:
Stellwagen E
Stellwagen E
中科院分区:
其他
文献类型:
--
作者:
Stellwagen NC;Stellwagen E

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DNA电泳迁移率高度依赖于分离发生的基质的性质。本文综述了在琼脂糖凝胶、聚丙烯酰胺凝胶和含有纠缠线性聚合物的溶液中,基质对DNA分离的影响,并将电泳迁移率与从其他类型的研究中获得的信息联系起来。DNA在各种筛分介质中的迁移率是由三个因素的相互作用决定的:DNA分子相对于基质有效孔径的相对大小,电场对基质的影响,以及电泳过程中DNA与基质的特定相互作用。
DNA electrophoretic mobilities are highly dependent on the nature of the matrix in which the separation takes place. This review describes the effect of the matrix on DNA separations in agarose gels, polyacrylamide gels and solutions containing entangled linear polymers, correlating the electrophoretic mobilities with information obtained from other types of studies. DNA mobilities in various sieving media are determined by the interplay of three factors: the relative size of the DNA molecule with respect to the effective pore size of the matrix, the effect of the electric field on the matrix, and specific interactions of DNA with the matrix during electrophoresis.
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