Surface modification of poly(dimethylsiloxane) for controlling biological cells' adhesion using a scanning radical microjet
Surface modification of poly(dimethylsiloxane) for controlling biological cells' adhesion using a scanning radical microjet
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DOI：
10.1016/j.tsf.2006.10.026
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发表时间：
2007-04-23
期刊：
影响因子：
2.1
通讯作者：
Ichiki, T.
中科院分区：
文献类型：
--
作者：
Tan, Helen M. L.;Fukuda, H.;Ichiki, T.
使用氧微等离子体的扫描自由基微射流（SRMJ）设备已被开发并成功应用于控制生物细胞在生物相容性聚合物材料聚（二甲基硅氧烷）（PDMS）上的附着。自由基微射流在局部、高速表面处理方面具有优势。此外，使用SRMJ的无掩模亲水图案化已被证明适用于图案化细胞培养，这在组织工程和基于细胞的生物传感器等新兴生物技术领域非常有用。由于控制 PDMS 表面特性是细胞附着不可或缺的先决条件，因此首先研究了氧气流量和处理时间对 PDMS 表面局部亲水图案的影响，以控制 HeLa 细胞（人上皮样癌细胞系）附着。基于使用原子力显微镜 (AFM) 和 X 射线光电子能谱 (XPS) 分析的表面特性，还讨论了处理过的 PDMS 薄膜的表面条件与附着细胞密度之间的关系。 (c) 2006 Elsevier B.V. 保留所有权利。
A scanning radical microjet (SRMJ) equipment using oxygen microplasma has been developed and successfully applied for controlling biological cells' attachment on biocompatible polymer material, poly(dimethylsiloxane) (PDMS). The radical microjet has advantages in localized and high-rate surface treatment. Moreover, maskless hydrophilic patterning using SRMJ has been demonstrated to be applicable to patterned cell cultivation which is useful in emerging biotechnological field such as tissue engineering and cell-based biosensors. Since control of PDMS surface properties is an indispensable prerequisite for cells' attachment, effects of oxygen flow rates and treatment time on localized hydrophilic patterning of PDMS surfaces were first investigated for controlling HeLa cells' (human epitheloid carcinoma cell line) attachment. Relationships between surface conditions of treated PDMS films and attached cell density are also discussed based on surface properties analyzed using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). (c) 2006 Elsevier B.V. All rights reserved.