New microsatellite markers for the rare plant Cercidiphyllum japonicum and their utility for Cercidiphyllum magnificum.
New microsatellite markers for the rare plant Cercidiphyllum japonicum and their utility for Cercidiphyllum magnificum.
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DOI：
10.3732/ajb.1000165
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发表时间：
2010-09
影响因子：
3
通讯作者：
Chen Chen-Chen;Yi-Hui Liu;C. Fu;Y. Qiu
中科院分区：
文献类型：
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作者：
Chen Chen-Chen;Yi-Hui Liu;C. Fu;Y. Qiu
为研究中国东部和日本本土濒危物种日本尾叶（Cercidiphyllum japonicum）的群体遗传学，建立了微卫星标记。方法与结果：采用Fast Isolation by AFLP of Sequences Containing Repeats （FIASCO）技术，对来自中国东部和日本两个种群的33个个体进行测试，成功扩增的12个微卫星标记显示多态性。总体而言，每个位点的等位基因数量在5到18之间。其中11个标记易扩增，且具有多态性。结论这些微卫星标记可以在精细和全范围的地理尺度上检测和表征子叶的群体遗传结构。
UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed for Cercidiphyllum japonicum to study population genetics of this endangered species native to both eastern China and Japan. • METHODS AND RESULTS Using the Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) protocol, 12 microsatellite markers that were successfully amplified showed polymorphism when tested on 33 individuals from two populations in eastern China and Japan. Overall, the number of alleles per locus ranged between 5 and 18. Eleven markers could be easily amplified and were polymorphic in C. magnificum. • CONCLUSIONS These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in Cercidiphyllum at fine and range-wide geographical scales.