ENUMERATION OF METHANOBREVIBACTER-SMITHII IN HUMAN FECES
ENUMERATION OF METHANOBREVIBACTER-SMITHII IN HUMAN FECES
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DOI：
10.1007/bf00451492
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发表时间：
1982-01-01
影响因子：
2.8
通讯作者：
WOLIN, MJ
中科院分区：
文献类型：
--
作者：
MILLER, TL;WOLIN, MJ
研制了一种含有头孢菌素和克林霉素的平板培养基来计数和分离人粪便中的产甲烷菌。产9CH4受试者的标本厌氧菌总数为1-8倍。1011/克干重。抗生素培养基产甲烷菌占厌氧菌总数的0.001~12.6%。年龄、性别或粪便干重百分比与产甲烷菌占总数的比例之间没有相关性。来自8个不产生甲烷的个体的样本中含有在抗生素培养基上产生非产甲烷菌落的细菌。手段。+-。每克干重总计数的对数的标准偏差为11.4±-。0.29和11.38+-。阳性组和阴性组分别为0.44。耐药菌群的数值为8.8.+-。1.13和7.78。+-。分别为1.08和1.08。从产生甲烷的受试者的每个标本的最稀释液中分离出产甲烷菌。所有分离株在形态、生理和免疫学上均与斯氏支原体完全相同。甲烷菌在基本上是CH4阴性粪便提取物的培养基中的生长表明，这些人的肠道内容物中没有营养物质或抑制物来阻止甲烷菌的生长。
A plating medium containing cephalothin and clindamycin was developed for enumeration and isolation of methanogens in human feces. Specimens from 9CH4-producing subjects had total anaerobe counts of 1-8 .times. 1011/g dry weight. Methanogen counts on the antibiotic medium ranged from 0.001-12.6% of the total anaerobe count. There was no correlation between age, sex or percent dry fecal weight and the ratio of methanogens to total counts. Specimens from 8 non-CH4-producing individuals contained bacteria that yielded nonmethanogenic colonies on the antibiotic medium. The means .+-. SD of the logarithm of the total counts per g dry weight were 11.4 .+-. 0.29 and 11.38 .+-. 0.44 for the positive and negative groups, respectively. Values for the antibiotic-resistant flora were 8.8 .+-. 1.13 and 7.78 .+-. 1.08, respectively. Methanogens were isolated from the most dilute inoculum of each specimen from CH4-producing subjects. All isolates were morphologically, physiologically and immunologically identical to M. smithii. Growth of methanogens in media that were essentially extracts of CH4-negative feces suggested that no nutrients were lacking or inhibitors present in intestinal contents that prevent the growth of methanogens in these individuals.