Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.
Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.
复制标题
逆转录病毒 pol 基因的计算机分析：将酶功能分配给特定序列以及与非病毒酶的同源性。
DOI：
10.1073/pnas.83.20.7648
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发表时间：
1986
影响因子：
11.1
通讯作者：
Doolittle,RF
中科院分区：
文献类型：
--
作者：
Johnson,MS;McClure,MA;Feng,DF;Gray,J;Doolittle,RF
A computer analysis of the amino acid sequences from the putative gene products of retroviral pol genes has revealed a 150-residue segment that is homologous with the ribonuclease H of Escherichia coli. The segment occurs at the carboxyl terminus of the region assigned to the 90-kDa reverse transcriptase polypeptide. In contrast, a section nearer the amino terminus of this sequence can be aligned with nonretroviral polymerases. The order of activities in the pol gene is thus: polymerase-ribonuclease-endonuclease. On another note, all retroviral endonuclease sequences contain a consensus zinc-binding "finger." This should not be confused with the well-known zinc requirement of reverse transcriptases.
