Presence of a functional TSH receptor on human erythrocytes
Presence of a functional TSH receptor on human erythrocytes
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DOI：
10.1016/j.biopha.2007.04.009
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发表时间：
2007-09-01
影响因子：
7.5
通讯作者：
Iervasi, Giorgio
中科院分区：
文献类型：
--
作者：
Balzan, Silvana;Nicolini, Giuseppina;Iervasi, Giorgio
促甲状腺激素受体(TSHR)已被报道存在于一些甲状腺外组织中，但其生理病理作用尚不清楚。促甲状腺激素(TSH)对体外培养的人红细胞Na+/K+-ATPase有一定的影响，但红细胞上的受体尚不清楚。本文观察了重组人TSH(RhTSH)对一组甲状腺摘除患者红细胞膜Na+/K+-ATPase活性的影响。H-3哇巴因结合实验显示，重组人促甲状腺激素可显著增加Na+/K+-ATPase的结合位点数(p=0.005)和Kd(p=0.006)。Rb-86测定的Na+/K+-ATPase活性未见明显差异。I-125-TSH结合实验和Western blotting结果表明，健康献血者红细胞中存在TSHR。结论：TSH对人红细胞Na~+/K~+-ATPase的作用可以通过TSHR的存在来解释。(C)2007年爱思唯尔·马森公司。版权所有。
The presence of thyrotropin receptors (TSHR) has been reported in some extrathyroidal tissues but its physio-pathological role still remains unclear. TSH (seems to) affects the human erythrocytes Na+/K+-ATPase in vitro, however receptors on erythrocytes have not yet been described.In this work the effect of recombinant human TSH (rhTSH) on sites number and activity of erythrocyte Na+/K+-ATPase was investigated in a group of thyroidectornized patient enrolled for rhTSH test. As detected by H-3-ouabain binding, rhTSH administration induced a significant increase in the number of sites (p = 0.005) and in the Kd (p = 0.006) of Na+/K+-ATPase. rhTSH did not induce significant difference in Na+/K+-ATPase activity measured by Rb-86 uptake.I-125-TSH binding studies and Western blotting data showed the existence of TSHR in the erythrocytes of healthy donors. In conclusion The TSH action on Na+/K+-ATPase of human erythrocytes can be explained by the presence of TSHR. (c) 2007 Elsevier Masson SAS. All rights reserved.