Setting Properties and Cytotoxicity Evaluation of a Premixed Bioceramic Root Canal Sealer
Setting Properties and Cytotoxicity Evaluation of a Premixed Bioceramic Root Canal Sealer
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DOI：
10.1016/j.joen.2011.01.003
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发表时间：
2011-05-01
影响因子：
4.2
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
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作者：
Loushine, Bethany A.;Bryan, Thomas E.;Tay, Franklin R.
简介：该研究调查了在不同水分含量（0-9重量%）存在下，预混合的基于磷酸硅酸钙的密封剂（EndoSequence BC Sealer;美国佐治亚州萨凡纳的Sealer）的凝固时间和显微硬度。使用产生最佳凝固特性的水分含量来制备EndoSequence BC密封剂套件，以与基于环氧树脂的密封剂（AH Plus; Dentsply Caulk，米尔福德，DE）进行细胞毒性比较。研究方法：用BC密封器、AH Plus、牙髓管密封器EWT（阳性对照）（SybronEndo，橙子CA）和Teflon（Small Parts Inc.，迈阿密湖泊，FL;阴性对照）。将细胞置于Transwell培养皿中，提供与MC 3 T3-E1细胞的间接接触。使用MTT（（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物）测定法在6周时间内评价细胞的琥珀酸脱氢酶活性。使用多项式回归模型拟合BC密封机和AH Plus的细胞毒性特征。使用Wald统计量分析50%细胞存活的时间（T-0.5），双侧显著性水平为0.05。结果如下：使用Gilmore针法，BC密封器需要至少168小时才能达到最终设置，并且当水包含在密封器中时，其微硬度显著下降（P = .004）。所有密封装置在24小时时均表现出严重的细胞毒性。AH Plus的细胞毒性逐渐降低并变为无细胞毒性，而BC密封器在6周内仍具有中度细胞毒性。BC密封器的T-0.5（5.10周; 95%置信区间[CI]，4.69-5.42，标准误[SE] = 0.09）和AK Plus的T-0.5（0.86周; 95% CI，0.68-1.05; SE = 0.18）之间存在显著差异（P <0.001）。结论：需要进一步的研究来评价BC封口机的凝固时间长度与其细胞毒性程度之间的相关性。（J Endod 2011;37：673-677）
Introduction: This study investigated the setting time and micohardness of a premixed calcium phosphate silicate based sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different moisture contents (0-9 wt%). The moisture content that produced the most optimal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity comparison with an epoxy resin based sealer (AH Plus; Dentsply Caulk, Milford, DE). Methods: Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50% of the cells to survive (T-0.5) was analyzed using the Wald statistic with a two-tailed significance level of 0.05. Results: BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the sealer (P = .004). All set sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became non-cytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference (P < .001) was detected between T-0.5 of BC Sealer (5.10 weeks; 95% confidence interval [CI], 4.69-5.42, standard error [SE] = 0.09) and T-0.5 of AK Plus (0.86 weeks; 95% CI, 0.68-1.05; SE = 0.18). Conclusions: Further studies are require I to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity. (J Endod 2011;37:673-677)