Vitellogenin fluctuations in haemolymph and fat body and dynamics of uptake into oöcytes during the reproductive cycle of Diploptera punctata
Vitellogenin fluctuations in haemolymph and fat body and dynamics of uptake into oöcytes during the reproductive cycle of Diploptera punctata
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DOI：
10.1016/0022-1910(81)90083-4
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发表时间：
1981
影响因子：
2.2
通讯作者：
E. Mundall;S. Tobe;B. Stay
中科院分区：
文献类型：
--
作者：
E. Mundall;S. Tobe;B. Stay
本文研究了斑点双翅蝗（Diploptera punctata）生殖周期中血淋巴和脂肪体可溶性蛋白滴度的变化，特别强调了卵黄蛋白原的产生及其在卵母细胞中的吸收。卵黄蛋白原首次检测到交配后2天的女性血淋巴成虫羽化在大约相同的时间，卵黄蛋白沉积在基础卵母细胞开始。第6天血淋巴卵黄原蛋白滴度达到峰值，与卵黄吸收的完成相关。此后，卵黄蛋白原水平下降，一般在整个妊娠期的大部分检测不到，再次上升前不久分娩与第二gonotrophic周期。总血淋巴蛋白水平与卵黄发生无关。交配雌性的可溶性脂肪体卵黄原蛋白滴度在第一个卵母细胞生长期保持较低水平，但在卵母细胞生长完成时上升几倍，并在整个妊娠和第二个性腺营养周期保持较高水平。总脂肪体可溶性蛋白质下降后，成人羽化与卵母细胞的增长。卵黄蛋白积累在基础卵母细胞的体积增加线性相关，直到开始的绒毛膜形成。因此，未检测到卵黄发生后生长期。
Haemolymph and fat body soluble protein titres have been examined during the reproductive cycle ofDiploptera punctata, with particular emphasis on the occurrence of vitellogenin and its uptake into the developing oöcytes. Vitellogenin was first detected in the haemolymph of mated females 2 days after adult eclosion at about the same time that vitellin deposition in basal oöcytes began. Peak haemolymph titres of vitellogenin occurred on day 6, correlated with the completion of yolk uptake. Thereafter vitellogenin levels declined and were generally undetectable throughout most of gestation, rising again shortly before parturition in association with the second gonotrophic cycle. Total haemolymph protein levels were not correlated with vitellogenesis.Soluble fat body vitellogenin titres of mated females remained low during the first oöcyte growth period but then rose several-fold at its completion and remained high throughout pregnancy and the second gonotrophic cycle. Total fat body soluble proteins decline after adult eclosion in association with oöcyte growth.Vitellin accumulation in basal oöcytes was related linearly to increase in volume until the onset of chorion formation. Thus no post-vitellogenic growth period was detected.