C-kit expression in germinoma: an imunohistochemistry-based study
C-kit expression in germinoma: an imunohistochemistry-based study
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DOI：
10.1007/s11060-005-1593-1
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发表时间：
2005-11-01
影响因子：
3.9
通讯作者：
Kuratsu, JI
中科院分区：
文献类型：
--
作者：
Nakamura, H;Takeshima, H;Kuratsu, JI
在我们的免疫组织化学研究中，我们对25例人原发颅内生殖细胞瘤和带有合胞滋养细胞巨细胞（STGC）的生殖细胞瘤进行了c-kit和胎盘碱性磷酸酶（PLAP）的染色。免疫组织化学表达采用半定量评分系统进行分级，其中3+ = 51-75%,4+ = 76-100%。25例中，7例（28%）c-kit评分为3+，18例（72%）c-kit评分为4+；8例（32%）PLAP评分为3+或4+。3例plap染色阴性均为强阳性，胚胎癌、未成熟畸胎瘤、卵黄囊瘤c-kit染色均为阴性。据报道，在生殖细胞瘤和伴STGC的生殖细胞瘤患者的脑脊液中可检测到c-kit的可溶性异构体（s-kit）。C-kit和s-kit可能是伴有或不伴有STGC的生殖细胞瘤的有效肿瘤标志物。
In our immunohistochemical study of 25 human primary intracranial germinomas and germinomas with syncytiotrophoblastic giant cells (STGC), we stained the same sections for c-kit and placental alkaline phosphatase (PLAP). Immunohistochemical expression was graded using a semi-quantitative scoring system where 3+ = 51-75%, and 4+ = 76-100%. Of the 25 cases, 7 (28%) were graded 3+ and 18 (72%) 4+ for c-kit; 8 (32%) were 3+ or 4+ for PLAP. All 3 cases negative for PLAP-staining were strongly positive and all embryonal carcinomas, immature teratomas, and yolk sac tumors were negative for c-kit staining. The soluble isoform of c-kit (s-kit) is reportedly detectable in cerebral spinal fluid of patients with germinomas and germinomas with STGC. C-kit and s-kit may be powerful tumor markers for germinomas with or without STGC.