Cloning of a novel orphan receptor (GCNF) expressed during germ cell development.
Cloning of a novel orphan receptor (GCNF) expressed during germ cell development.
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DOI：
10.1210/mend.8.10.7854358
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发表时间：
1994-10
影响因子：
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通讯作者：
Fang Chen;A. J. Cooney;Yaolin Wang;Simon W. Law;B. W. O'malley
中科院分区：
文献类型：
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作者：
Fang Chen;A. J. Cooney;Yaolin Wang;Simon W. Law;B. W. O'malley
我们已经克隆了核受体超家族的一个新成员，该成员是通过低严格杂交策略从小鼠组织的互补DNA文库中鉴定出来的。推导的蛋白质序列由495个氨基酸组成，由核受体超家族特有的DNA结合域和配体结合域组成。这个新孤儿的初级序列与以前克隆的成员和亚群的序列不同。体外合成的蛋白的DNA结合特性分析表明，这个新的孤儿受体与TCAAGGTCA序列结合，TCAAGGTCA序列包括类固醇生成因子-1的半个位点和具有0个碱基间隔的直接重复序列。Northern印迹和核糖核酸酶保护分析表明，该受体主要在睾丸中表达。原位杂交实验结果证实了这一观察结果，并表明它位于生精细胞中。卵巢发育中的卵母细胞也有高水平的表达。因此，该基因的高水平表达仅限于发育中的生殖细胞、卵母细胞和生精细胞。我们推测这种孤儿受体可能是一个参与调节减数分裂某些方面的分子，该因子的主要功能可能参与了配子发生过程中生殖细胞发育过程中基因表达的调节。它被指定为生殖细胞核因子。
We have cloned a novel member of the nuclear receptor superfamily that has been identified from complementary DNA libraries derived from mouse tissues using a low stringency cross hybridization strategy. The deduced protein sequence contains 495 amino acids and consists of the characteristic DNA-binding and ligand-binding domains of the nuclear receptor superfamily. The primary sequence of this new orphan is distinct from those of previously cloned members and subgroups. Analysis of the DNA-binding properties of the in vitro synthesized protein revealed that this new orphan receptor binds to the sequence TCAAGGTCA that includes the steroidogenic factor-1 half-site and direct repeat with 0 bp spacing elements. Northern blot and ribonuclease protection assays showed that the receptor was predominantly expressed in the testis. Results from in situ hybridization experiments confirmed this observation and showed it to be located in the spermatogenic cells. High level expression was also detected in developing oocytes in the ovary. Thus, high level expression of this gene is restricted to developing germ cells, the oocytes and spermatogenic cells. We speculate that this orphan receptor may be a molecule involved in regulating some aspect of meiosis, and that the major function of this factor is likely to be involved in the regulation of gene expression in germ cell development during gametogenesis. It has been designated germ cell nuclear factor.