RAB6 and microtubules restrict protein secretion to focal adhesions
RAB6 and microtubules restrict protein secretion to focal adhesions
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DOI：
10.1083/jcb.201805002
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发表时间：
2019-07-01
影响因子：
7.8
通讯作者：
Miserey-Lenkei, Stephanie
中科院分区：
文献类型：
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作者：
Fourriere, Lou;Kasri, Amal;Miserey-Lenkei, Stephanie
为了确保它们的稳态和维持分化的功能，细胞连续地将不同的货物运输到各种细胞区室，特别是运输到细胞表面。分泌的蛋白质在沿着微管轨道到达质膜之前沿着细胞内路线从内质网通过高尔基复合体运输。使用同步分泌测定，我们在这里报告，胞吐不随机发生在细胞表面，但在本地化的热点并列的焦点粘连。虽然微管参与其中，但RAB6依赖性机制起着至关重要的作用。我们观察到，无论运输的货物，大多数后高尔基载体是阳性的RAB 6和它的失活导致广泛的蛋白质分泌减少。因此，RAB6可能是高尔基体后分泌的一般调节剂。
To ensure their homeostasis and sustain differentiated functions, cells continuously transport diverse cargos to various cell compartments and in particular to the cell surface. Secreted proteins are transported along intracellular routes from the endoplasmic reticulum through the Golgi complex before reaching the plasma membrane along microtubule tracks. Using a synchronized secretion assay, we report here that exocytosis does not occur randomly at the cell surface but on localized hotspots juxtaposed to focal adhesions. Although microtubules are involved, the RAB6-dependent machinery plays an essential role. We observed that, irrespective of the transported cargos, most post-Golgi carriers are positive for RAB6 and that its inactivation leads to a broad reduction of protein secretion. RAB6 may thus be a general regulator of post-Golgi secretion.