Activation of Aicda gene transcription by Pax5 in plasmacytoma cells.
Activation of Aicda gene transcription by Pax5 in plasmacytoma cells.
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DOI：
10.1007/s12026-012-8357-8
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发表时间：
2013-03
影响因子：
4.4
通讯作者：
Hagman, James
中科院分区：
文献类型：
--
作者：
Dege, Carissa;Hagman, James
Activation Induced Deaminase (AID) is an enzyme responsible for somatic hypermutation (SHM) and immunoglobulin heavy chain class switch recombination (CSR). Because AID causes double-stranded breaks in DNA, its expression is highly regulated and is normally restricted to germinal center B cells. Dysregulated AID expression can lead to cancer as a result of AID-mediated chromosomal translocations. Many transcription factors including Paired box protein 5 (Pax5) have been implicated in regulating the expression of Aicda, the gene encoding AID. In this study, we demonstrate that exogenous expression of Pax5 in a murine plasmacytoma cell line, 558LμM, leads to robust activation of endogenous Aicda transcription. Pax5 is known to initiate transcription both through its N-terminal paired DNA-binding domain (DBD) and C-terminal-activation domain. Through mutational analysis, we demonstrate that Pax5 regulates Aicda transcription through its C-terminal-activation domain. Together, our work describes a novel system that will be useful for determining how Pax5 regulates Aicda transcription.
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