Expansion, retention and loss in the Acyl-CoA synthetase "Bubblegum" (Acsbg) gene family in vertebrate history
Expansion, retention and loss in the Acyl-CoA synthetase "Bubblegum" (Acsbg) gene family in vertebrate history
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DOI：
10.1016/j.gene.2018.04.058
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发表时间：
2018-07-20
期刊：
影响因子：
3.5
通讯作者：
Castro, L. Filipe C.
中科院分区：
文献类型：
--
作者：
Lopes-Marques, Monica;Machado, Andre M.;Castro, L. Filipe C.
脂肪酸（FA）在所有脂质分子中占相当大的比例，在许多生理过程中发挥着重要作用。在动物中，大多数复杂的脂质分子源自 FA 通过多种生化途径的转化。然而，对于 FA 来说，要加入这些途径，它们需要一个激活步骤。 FA 激活是由酰基辅酶 A 合酶的限速作用催化的。先前已经描述了几个酰基辅酶A酶家族，并根据它们处理的FA的链长度进行分类。在这里，我们探讨了 ACSBG 基因家族的进化历史，该基因家族激活具有 > 16 个碳的 FA。目前，脊椎动物中有两个不同的 ACSBG 基因家族：ACSBG1 和 ACSBG2。我们提供的证据表明，脊椎动物谱系中存在更广泛且不平等的 ACSBG 基因库。我们鉴定了一种新的类 ACSBG 基因谱系，该谱系特异存在于两栖动物、射线鳍鱼、腔棘鱼和软骨鱼中，名为 ACSBG3。此外，我们还发现 ACSBG2 基因谱系在 Theria 祖先中复制。因此，我们的研究结果为脊椎动物的 FA 激活提供了更丰富的理解，并为比较和功能分析的相关性提供了关键见解，以感知生理差异，即与脂质代谢途径相关的生理差异。
Fatty acids (FAs) constitute a considerable fraction of all lipid molecules with a fundamental role in numerous physiological processes. In animals, the majority of complex lipid molecules are derived from the transformation of FAs through several biochemical pathways. Yet, for FAs to enroll in these pathways they require an activation step. FA activation is catalyzed by the rate limiting action of Acyl-CoA synthases. Several Acyl-CoA enzyme families have been previously described and classified according to the chain length of FAs they process. Here, we address the evolutionary history of the ACSBG gene family which activates, FAs with > 16 carbons. Currently, two different ACSBG gene families, ACSBG1 and ACSBG2, are recognized in vertebrates. We provide evidence that a wider and unequal ACSBG gene repertoire is present in vertebrate lineages. We identify a novel ACSBG-like gene lineage which occurs specifically in amphibians, ray finned fishes, coelacanths and cartilaginous fishes named ACSBG3. Also, we show that the ACSBG2 gene lineage duplicated in the Theria ancestor. Our findings, thus offer a far richer understanding on FA activation in vertebrates and provide key insights into the relevance of comparative and functional analysis to perceive physiological differences, namely those related with lipid metabolic pathways.