Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients
Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients
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DOI：
10.1007/s00404-017-4317-9
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发表时间：
2017-04-01
影响因子：
2.6
通讯作者：
Amidi, Fardin
中科院分区：
文献类型：
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作者：
Nekoonam, Saeid;Naji, Mohammad;Amidi, Fardin
目的AKT在颗粒叶黄素细胞（GC）增殖和卵泡形成中起关键作用，AKT与雄激素之间存在相互反馈作用。因此，我们旨在评估AKTs在高雄激素（+HA） PCOS患者GCs中的作用。方法将PCOS分为对照组、+HA PCOS和-HA（非高雄激素型）PCOS。所有组均接受GnRH拮抗剂方案刺激排卵。从大卵泡中抽取卵泡液，用细胞过滤法分离GCs。采用实时荧光定量PCR （qRT-PCR）分析AKT1、AKT2、AKT3和雄激素受体（AR） mRNA的表达，采用western blotting检测总akt和p-AKT （Ser（473）和Thr(308)）的表达。结果+HA PCOS组AKT1、AKT2、AR mRNA和p- akt蛋白的表达均较高（p < 0.05）
Purpose AKTs have a pivotal role in the granulosa-lutein cell (GC) proliferation and folliculogenesis, and there is a reciprocal feedback between AKT with androgen. Therefore, we aimed to evaluate the role of AKTs in GCs of hyperandrogenic (+HA) PCOS cases.Method There were three groups: control, +HA PCOS and -HA (non-hyperandrogenic) PCOS. All groups were subjected to GnRH antagonist protocol for stimulation of ovulation. Follicular fluid was aspirated from large follicles, and GCs were isolated using cell strainer method. AKT1, AKT2, AKT3, and androgen receptor (AR) mRNA expressions were analyzed with quantitative real-time PCR (qRT-PCR), and total-AKT and p-AKT (Ser(473) & Thr(308)) were investigated using western blotting.Results There were high levels of AKT1, AKT2, and AR mRNA expressions and high levels of p-AKT protein expression in the +HA PCOS group (p