THE ESSENTIAL BACTERIAL CELL-DIVISION PROTEIN FTSZ IS A GTPASE
THE ESSENTIAL BACTERIAL CELL-DIVISION PROTEIN FTSZ IS A GTPASE
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DOI：
10.1038/359254a0
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发表时间：
1992-09-17
期刊：
影响因子：
64.8
通讯作者：
ROTHFIELD, L
中科院分区：
文献类型：
--
作者：
DEBOER, P;CROSSLEY, R;ROTHFIELD, L
细胞分裂定义了分裂周期的最后阶段，在此阶段细胞收缩导致子细胞的形成。负责这一过程的生化机制知之甚少。在细菌中，ftsZ基因产物FtsZ是细胞分裂所必需的1 - 3，17，在胞质分裂中起着重要作用。FtsZ的细胞浓度调节分裂频率4，遗传研究表明它是几种内源性分裂因子的靶点5。在中隔内陷开始时，FtsZ蛋白从细胞质募集到分裂位点，在那里它组装成一个环，该环保持与内陷中隔的前缘相关联，直到中隔完成6。在这里，我们报告说，FtsZ特异性结合和水解GTP。该反应可以分解成一个GTP依赖性的激活阶段，这是显着影响FtsZ的浓度，和一个水解阶段，其中GTP被水解为GDP。结果表明，GTP的结合和水解是重要的，使FtsZ支持细菌胞质分裂，无论是通过促进组装的FtsZ环和/或通过催化细胞动力学过程本身的一个重要步骤。
CYTOKINESIS defines the last stage in the division cycle, in which cell constriction leads to the formation of daughter cells. The biochemical mechanisms responsible for this process are poorly understood. In bacteria, the ftsZ gene product, FtsZ, is required for cell division1-3,17, playing a prominent role in cytokinesis. The cellular concentration of FtsZ regulates the frequency of division4 and genetic studies have indicated that it is the target of several endogenous division inhibitors5. At the time of onset of septal invagination, the FtsZ protein is recruited from the cytoplasm to the division site, where it assembles into a ring that remains associated with the leading edge of the invaginating septum until septation is completed 6. Here we report that FtsZ specifically binds and hydrolyses GTP. The reaction can be dissociated into a GTP-dependent activation stage that is markedly affected by the concentration of FtsZ, and a hydrolysis stage in which GTP is hydrolysed to GDP. The results indicate that GTP binding and hydrolysis are important in enabling FtsZ to support bacterial cytokinesis, either by facilitating the assembly of the FtsZ ring and/or by catalysing an essential step in the cytokinetic process itself.