Ultrafiltration is theoretically equivalent to equilibrium dialysis but much simpler to carry out.
Ultrafiltration is theoretically equivalent to equilibrium dialysis but much simpler to carry out.
复制标题
超滤在理论上等同于平衡透析，但执行起来更简单。
DOI：
10.1016/0003-9861(78)90015-2
复制
发表时间：
1978
影响因子：
3.9
通讯作者：
W. Cropper
中科院分区：
文献类型：
--
作者：
J. Sophianopoulos;S. J. Durham;A. J. Sophianopoulos;H. L. Ragsdale;W. Cropper
This study shows that if one component of a reaction at equilibrium is freely diffusible through a semipermeable membrane, and if an aliquot of this component is removed through the membrane at its equilibrium concentration, the concentration of this component in the reaction mixture remains unchanged. This is illustrated by the binding of manganese (Mn2+) to concanavalin A. It is also shown that, at concentrations of calcium ions near saturation levels (0.01mCaCh2, pH 5.2, 0.2mNaCl), the binding of 1 mol of Mn2+is extremely strong, with a dissociation constantK< 10−7, and at least 1 additional mol of Mn2+/mol of concanavalin A binds less strongly. As the pH is lowered, the affinity decreases to a small extent, until at pH 1.82 approximately 0.25 mol of Mn2+binds/mol of protein. A possible application of the method to measure binding as a function of ligand concentration is described.
