Graphene oxide with in-situ grown Prussian Blue as an electrochemical probe for microRNA-122
Graphene oxide with in-situ grown Prussian Blue as an electrochemical probe for microRNA-122
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DOI：
10.1007/s00604-018-3204-9
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发表时间：
2019-01
影响因子：
5.7
通讯作者：
Feng Wang;Yaru Chu;Yijing Ai;Lin Chen;F. Gao
中科院分区：
文献类型：
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作者：
Feng Wang;Yaru Chu;Yijing Ai;Lin Chen;F. Gao
基于氧化石墨烯（GO）直接生长电活性普鲁士蓝（PB），构建了microRNA电化学生物传感器。首先将与肝细胞癌生物标志物microRNA-122互补的巯基修饰的探针DNA锚定在金电极（AuE）上。然后，GO（具有大的表面和多个活性位点）通过π相互作用吸附在探针DNA上。随后，PB纳米粒子直接生长在GO上通过交替浸渍在溶液中的FeCl 3和六氰基铁（III）的电极。在将所得电极与微小RNA-122溶液孵育后，电极上的探针DNA与微小RNA-122相互作用以形成刚性双链体。这导致电活性PB/GO从感测界面的释放和电流的降低，通常在0.18 V（相对于Ag/AgCl（3 M KCl））下测量。该传感器覆盖10 fM至10 nM microRNA-122浓度范围，检测限为1.5 fM。该方法已成功应用于真实的生物样品中microRNA-122的测定。图示摘要氧化石墨烯-普鲁士蓝原位生长法作为电化学探针用于microRNA-122的分析。
An electrochemical biosensor for microRNA was constructed on the basis of direct growth of electroactive Prussian Blue (PB) on graphene oxide (GO). A mercapto-modified probe DNA that is complementary to the hepatocellular carcinoma biomarker microRNA-122 was firstly anchored on a gold electrode (AuE). Then, GO (with its large surface and multiple active sites) was adsorbed on probe DNA through π-interaction. Subsequently, the PB nanoparticles were directly grown on GO via alternative dipping the electrode in solutions of FeCl3and hexacyanoferrate(III). Upon incubation of the resulting electrode with a solution of microRNA-122, the probe DNA on the electrode interacts with microRNA-122 to form a rigid duplex. This results in the release of electroactive PB/GO from the sensing interface and a decrease in current, typically measured at 0.18 V (vs. Ag/AgCl (3 M KCl)). The sensor covers the 10 fM to 10 nM microRNA-122 concentration range and has a 1.5 fM detection limit. The method was successfully applied to the determination of microRNA-122 in real biological samples.Graphical abstractGraphene oxide with in-situ grown Prussian Blue is applied as an electrochemical probe for the analysis of microRNA-122.