Metabolomics of Volatile Organic Compounds in Cystic Fibrosis Patients and Controls
Metabolomics of Volatile Organic Compounds in Cystic Fibrosis Patients and Controls
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DOI：
10.1203/00006450-201011001-00143
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Dompeling, Edward
中科院分区：
文献类型：
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作者：
Robroeks, Charlotte M. H. H. T.;van Berkel, Joep J. B. N.;Dompeling, Edward
在囊性纤维化 (CF) 中，气道炎症会导致活性氧产生增加，从而导致细胞膜降解。在此过程中，会形成挥发性有机化合物 (VOC)。测量 CF 患者呼出气中的 VOC 可能有助于评估气道炎症。本研究调查 VOC 的“代谢组学”是否可以区分 CF 和对照，以及有和没有假单胞菌定植的 CF 患者。本研究纳入了 105 名儿童（48 名 CF 患者，57 名对照）。收集呼出气后，将样本转移到含有活性炭的试管中，以吸附和稳定 VOC。通过气相色谱-飞行时间质谱法对样本进行分析，以评估 VOC 特征。分析显示，第 1099 章通过使用 22 种 VOC，可以对 10 种 VOC 进行 100% 的正确识别，1 小时间隔内 VOC 测量的再现性非常好（匹配因子 0.90 +/- 0.038）。这种新技术不仅能够区分 CF 患者和对照，还能够区分有或没有假单胞菌定植的 CF 患者 (Pediatr Res 68: 75-80, 2010)。
In cystic fibrosis (CF), airway inflammation causes an increased production of reactive oxygen species, responsible for degradation of cell membranes. During this process, volatile organic compounds (VOCs) are formed. Measurement of VOCs in exhaled breath of CF patients may be useful for the assessment of airway inflammation. This study investigates whether "metabolomics' of VOCs could discriminate between CF and controls, and between CF patients with and without Pseudomonas colonization. One hundred five children (48 with CF, 57 controls) were included in this study. After exhaled breath collection, samples were transferred onto tubes containing active carbon to adsorb and stabilize VOCs. Samples were analyzed by gas chromatography-time of flight-mass spectrometry to assess VOC profiles. Analysis showed that 1099 VOCs had a prevalence of at least 7%. By using 22 VOCs, a 100% correct identification of CF patients and controls was possible. With 10 VOCs, 92% of the subjects were correctly classified. The reproducibility of VOC measurements with a 1-h interval was very good (match factor 0.90 +/- 0.038). We conclude that metabolomics of VOCs in exhaled breath was possible in a reproducible way. This new technique was able to discriminate not only between CF patients and controls but also between CF patients with or without Pseudomonas colonization. (Pediatr Res 68: 75-80, 2010)