Haptoglobins as markers of blood-CSF barrier dysfunction: the findings in normal CSF
Haptoglobins as markers of blood-CSF barrier dysfunction: the findings in normal CSF
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DOI：
10.1016/s0022-510x(00)00461-5
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发表时间：
2001-01-01
影响因子：
4.4
通讯作者：
Thompson, EJ
中科院分区：
文献类型：
--
作者：
Chamoun, V;Zeman, A;Thompson, EJ
39名健康人的脑脊液显示，随着年龄的增长，血-脑脊液屏障通透性增加，并证实结合珠蛋白比总蛋白更敏感，但预测屏障通透性的能力较差。结合珠蛋白(Hp)用聚丙烯酰胺凝胶电泳法和免疫印迹法鉴定。Hp1-1(35埃，85 kDa)在所有(9/9)表现出该表型的受试者中检出。以Hp 2-1为表型的人群中，53%(8/15)检出Hp 2-1(42埃，120 kDa)。HP2-2(54埃，160 kDa)仅在20%(3/15)的HP2-2表型患者中检出。检测到任何结合珠蛋白物种的可能性与分子大小和屏障提供的抵抗力相对应。在年龄小于或等于45岁的年轻受试者中，两个较小的物种Hp1-1和Hp2-1之间的发病率存在显著差异。然而，在45岁的人群中，发病率在两个较大的物种Hp 2-1和Hp 2-2之间存在显著差异。在Hp 2-1表型者中，高龄组的检出率较高。结合珠蛋白随年龄增加的可能性与屏障功能随年龄增长而受损的观点一致，也表明HP2-1和HP2-2是屏障功能的敏感标志物。在正常脑脊液中可察觉到结合珠蛋白的出现，即使在较大的物种中也是如此，这表明人们对它们的存在意味着屏障损伤持保留态度。(C)2001 Elsevier Science B.V.保留所有权利。
Cerebrospinal fluid from 39 healthy individuals showed evidence for increasing blood-CSF barrier permeability with age, and confirmed that haptoglobins are more sensitive but less predictive markers of barrier permeability than total protein. Haptoglobin (Hp) species were identified by polyacrylamide gel electrophoresis followed by immunoblotting. Hp 1-1 (35 Angstrom, 85 kDa) was detected in all (9/9) subjects who exhibited this phenotype. Hp 2-1 (42 Angstrom, 120 kDa) was detected in 53% (8/15) of subjects in whom Hp 2-1 was the phenotype. Hp 2-2 (54 Angstrom, 160 kDa) was detected in only 20% (3/15) of subjects who exhibited this phenotype. The likelihood of detecting any haptoglobin species corresponded to the molecular size and the consequent resistance offered by the barrier. Among younger subjects aged less than or equal to 45 years, a significant difference in incidence occurred between the two smaller species Hp 1-1 and Hp 2-1. However, among those aged >45, the significant difference in incidence was between the two larger species Hp 2-1 and Hp 2-2. The incidence of detection among those with Hp 2-1 phenotypes was higher in the older age group. The increased likelihood of detecting haptoglobins with age is in keeping with the notion that barrier function is compromised by age, and also indicates that Hp 2-1 and Hp 2-2 are sensitive markers of barrier function. The appreciable incidence of haptoglobins in normal CSF, even of the larger species, suggests reservation in assuming that their presence signifies barrier damage. (C) 2001 Elsevier Science B.V. All rights reserved.