Dicer is essential for mouse development
Dicer is essential for mouse development
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DOI：
10.1038/ng1253
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发表时间：
2003-11-01
期刊：
影响因子：
30.8
通讯作者：
Hannon, GJ
中科院分区：
文献类型：
--
作者：
Bernstein, E;Kim, SY;Hannon, GJ
为了解决哺乳动物中RNA干扰(RNAi)相关途径的生物学功能，我们在小鼠中中断了Dicer1基因。Dicer1基因的缺失会导致发育早期的致命性死亡，Dicer1基因缺失的胚胎会耗尽干细胞。再加上我们无法产生可存活的Dicer1缺失的胚胎干细胞，这表明在小鼠发育的早期，迪格尔，以及暗示的RNAi机制，在维持干细胞数量方面发挥了作用。
To address the biological function of RNA interference (RNAi) related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1 - null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1 - null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.