Apoptosis Induction in Cancer Cells by Ultrasound Exposure
Apoptosis Induction in Cancer Cells by Ultrasound Exposure
复制标题
超声波照射诱导癌细胞凋亡
DOI：
10.1143/jjap.43.3245
复制
发表时间：
2004
影响因子：
1.5
通讯作者：
S. Takeuchi
中科院分区：
文献类型：
--
作者：
A. Watanabe;K. Kawai;Toshio Sato;H. Nishimura;N. Kawashima;S. Takeuchi
研究超声辐照抑制肿瘤细胞增殖的方法，以开发一种新的微创肿瘤治疗方法。将带有螺栓夹持Langevin型传感器（BLT）的不锈钢隔膜连接到本研究中使用的超声暴露系统中水箱的底部。将烧瓶中的小鼠T淋巴瘤（EL-4）的癌细胞在暴露时间、超声波频率、超声波波形等的各种条件下暴露于超声波。在培养过程中，暴露于超声波的癌细胞数量减少。本研究通过电泳、酶活性测定和形态学观察证实了超声辐照能诱导癌细胞凋亡，从而抑制癌细胞增殖。
The methods of suppressing cancer cell proliferation by ultrasound exposure were investigated to develop a new minimally invasive cancer treatment. A stainless-steel diaphragm with a bolt-clamped Langevin-type transducer (BLT) was attached to the bottom of a water tank in the ultrasound exposure system used in this study. Cancer cells of a mouse T lymphoma (EL-4) in a flask were exposed to ultrasound under various conditions of exposure time, ultrasound frequency, ultrasound waveform, and so forth. The number of cancer cells exposed to ultrasound decreased during the culturing process. In this study, it was proved by electrophoresis, enzyme activity measurement and morphological observation that cancer cell proliferation can be suppressed by apoptosis induction in cancer cells by ultrasound exposure.