Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus
Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus
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DOI：
10.1111/j.1462-2920.2011.02514.x
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发表时间：
2012-02-01
影响因子：
5.1
通讯作者：
Scanlan, David J.
中科院分区：
文献类型：
--
作者：
Mazard, Sophie;Ostrowski, Martin;Scanlan, David J.
保守的标记，如16S rRNA基因不提供足够的分子分辨率，以确定空间结构的海洋聚球藻种群，或适应不同的生态位的“生态型”。多位点序列分析，针对七个“核心”基因的分类解决聚球藻菌株和相关的以前的系统发育分析，包括一系列的标记。尽管公认的重要性，横向基因转移在塑造海洋蓝藻的基因组，多位点序列分析120多个菌株反映了克隆种群结构的主要谱系和亚群。选择了一个单一的核心基因组位点petB，编码细胞色素B（6）f复合物的细胞色素B（6）亚基，以扩大我们对海洋聚球藻种群多样性和生态学的理解。从对比网站克隆的环境petB序列突出了许多遗传和生态上不同的集群，其中一些代表新的，环境丰富的分支没有培养的代表。为了扩展生态分析，petB的短序列，分类分辨率和准确的自动比对非常适合高通量和高分辨率测序项目，以探索海洋聚球藻的生态学，进化和生物学之间的联系。
Conserved markers such as the 16S rRNA gene do not provide sufficient molecular resolution to identify spatially structured populations of marine Synechococcus, or 'ecotypes' adapted to distinct ecological niches. Multi-locus sequence analysis targeting seven 'core' genes was employed to taxonomically resolve Synechococcus isolates and correlate previous phylogenetic analyses encompassing a range of markers. Despite the recognized importance of lateral gene transfer in shaping the genomes of marine cyanobacteria, multi-locus sequence analysis of more than 120 isolates reflects a clonal population structure of major lineages and subgroups. A single core genome locus, petB, encoding the cytochrome b(6) subunit of the cytochrome b(6)f complex, was selected to expand our understanding of the diversity and ecology of marine Synechococcus populations. Environmental petB sequences cloned from contrasting sites highlight numerous genetically and ecologically distinct clusters, some of which represent novel, environmentally abundant clades without cultured representatives. With a view to scaling ecological analyses, the short sequence, taxonomic resolution and accurate automated alignment of petB is ideally suited to high-throughput and high-resolution sequencing projects to explore links between the ecology, evolution and biology of marine Synechococcus.