Shigella flexneri O-antigens revisited: final elucidation of the O-acetylation profiles and a survey of the O-antigen structure diversity
Shigella flexneri O-antigens revisited: final elucidation of the O-acetylation profiles and a survey of the O-antigen structure diversity
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DOI：
10.1111/j.1574-695x.2012.01000.x
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发表时间：
2012-11-01
影响因子：
--
通讯作者：
Knirel, Yuriy A.
中科院分区：
文献类型：
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作者：
Perepelov, Andrei V.;Shekht, Maria E.;Knirel, Yuriy A.
福氏志贺氏菌是引起志贺氏菌病的重要人类病原体。弗氏沙门氏菌在血清学上是异质的，根据o抗原，目前分为14种。自20世纪60年代以来，对福氏葡萄球菌o抗原（o -多糖）的结构进行了研究，但仍有一些空白。在这项工作中，利用一维和二维1H-和13C-NMR，重新研究了几种flexneri类型的o-多糖，并确定了它们的结构（类型2b， 3b, 3c, 5b， X）或根据o-乙酰化模式进行了修改（类型3a， Y, 6, 6a）。结果，在所有之前没有详细研究过的o -多糖中确定了o -乙酰化位点，从而完成了对flexneri型菌株o -抗原结构的长期阐明。根据志贺氏菌群内o抗原的血清学和遗传关系以及福氏菌与大肠杆菌之间的关系，总结和讨论了有关福氏菌o抗原结构的最新和已发表的资料。
Shigella flexneri is an important human pathogen causing shigellosis. Strains of S. flexneri are serologically heterogeneous and, based on O-antigens, are currently classified into 14 types. Structures of the O-antigens (O-polysaccharides) of S. flexneri have been under study since 1960s but some gaps still remained. In this work, using one- and two-dimensional 1H- and 13C-NMR spectroscopy, the O-polysaccharides of several S. flexneri types were reinvestigated, and their structures were either confirmed (types 2b, 3b, 3c, 5b, X) or amended in respect to the O-acetylation pattern (types 3a, Y, 6, 6a). As a result, the O-acetylation sites were defined in all O-polysaccharides that had not been studied in detail earlier, and the long story of S. flexneri type strain O-antigen structure elucidation is thus completed. New and published data on the S. flexneri O-antigen structures are summarized and discussed in view of serological and genetic relationships of the O-antigens within the Shigella group and between S. flexneri and Escherichia coli.