Identification and Fine Mapping of qCTH4, a Quantitative Trait Loci Controlling the Chlorophyll Content from Tillering to Heading in Rice (Oryza sativa L.)
Identification and Fine Mapping of qCTH4, a Quantitative Trait Loci Controlling the Chlorophyll Content from Tillering to Heading in Rice (Oryza sativa L.)
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DOI：
10.1093/jhered/ess041
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发表时间：
2012-09-01
影响因子：
3.1
通讯作者：
Li, Yuhua
中科院分区：
文献类型：
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作者：
Jiang, Shukun;Zhang, Xijuan;Li, Yuhua
叶绿素含量是育种者选择的重要性状之一，受水稻自然变异产生的数量性状基因座(QTL)控制。利用丽江新探黑谷(LTH)和沈农265(SN265)两个杂交组合的94个重组自交系，对控制叶绿素含量的QTL进行了分析。共检测到22个控制分蘖期、抽穗期和成熟期叶绿素含量的QTL。其中，莱斯cv.在第4染色体上只有1个座位(QCTH4)有一个正等位基因。进一步分析表明，qCTH4的遗传效应是从分蘖到抽穗期间的净效应。控制分蘖至抽穗期间叶绿素含量的QTLqCTH4位于第4染色体RM255~RM349之间，LOD值为19.41，解释了61.42%的表型变异。为了消除其他QTL的影响，根据114个SSR标记的基因型别，筛选出1个残留杂合子植株RH-qCTH4。利用RH-qCTH4通过自交获得的分离群体，将该区域缩小到RM3276和RM17494之间的大约771kb的靶区。这些结果为qCTH4的图位克隆和高效光效品种的分子标记辅助选择奠定了基础。
The chlorophyll content is one of the most important traits selected by breeders, and it is controlled by quantitative trait loci (QTLs) derived from natural variations in rice. We analyzed the QTL controlling chlorophyll content by using 94 RILs derived from a cross between 2 japonica rice cultivars, Lijiangxintuanheigu (LTH) and Shennong265 (SN265). Twenty-two QTLs controlling chlorophyll content at tillering stage, heading stage, and maturity stage were detected, respectively. Among them, Rice cv. LTH had a positive allele only at 1 locus (qCTH4) on chromosome 4. Further analysis indicated that the genetic effect of qCTH4 was the net effects within the period from tillering to heading. The QTL qCTH4 controlling chlorophyll content from tillering to heading locates between RM255 and RM349 on chromosome 4 with a LOD score 19.41, and the QTL qCTH4 explains 61.42% of phenotypic variation. In order to eliminate the influence of other QTLs, 1 single residual heterozygous plant, RH-qCTH4, was selected based on the genotypes of 114 Simple Sequence Repeat (SSR) markers. Using the segregating population derived from RH-qCTH4 by self-crossing, this region was narrowed down to an interval between RM3276 and RM17494 in an approximately 771 kb target region. These results are useful for map-based cloning of qCTH4 and for marker-assisted selection of high photosynthetic efficiency variety.