Tissue diffusion and retention of metalloproteinases in ascending aortic aneurysms and dissections
Tissue diffusion and retention of metalloproteinases in ascending aortic aneurysms and dissections
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DOI：
10.1016/j.humpath.2008.08.002
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发表时间：
2009-03-01
期刊：
影响因子：
3.3
通讯作者：
Michel, Jean-Baptiste
中科院分区：
文献类型：
--
作者：
Borges, Luciano F.;Touat, Ziad;Michel, Jean-Baptiste
人类动脉瘤和胸升主动脉夹层的组织学改变包括中层内的粘液样变性区域，与细胞消失和细胞外基质弹性和胶原纤维破坏的区域共定位。我们研究了基质金属蛋白酶的存在与其通过组织扩散或保留在动脉瘤和升主动脉夹层粘液变性区域的能力。对手术时收集的9例对照组、33例动脉瘤患者和14例急性夹层患者的上行动脉瘤进行了分析。无论病理性前列腺炎的病因或表型表达如何，形态学方面都是相似的，涉及与粘液变性相关的细胞外基质分解和细胞稀疏区域。基质金属蛋白酶原-2的释放，组成型表达的平滑肌细胞，是没有什么不同的控制和血管平滑肌瘤，而血管平滑肌瘤释放更多的活性形式。基质金属蛋白酶原9和活性基质金属蛋白酶9的释放在对照组和囊性动脉瘤组之间也相似。MMP-2和MMP-9的免疫组化染色在对照组和病理组中均较弱。相反，释放的MMP-7（基质溶解素）和MMP-3（基质溶解素-1）不能检测到的条件培养基中，但存在于组织提取物中，没有检测到的定量差异之间的控制和病理性乳腺癌。MMP-7和MMP-3的免疫组化染色显示其保留在粘液变性的领域，和免疫染色的半定量评价显示更多的MMP-7在病理性乳腺癌比对照组。总之，粘液变性区域，动脉瘤的标志，胸升支动脉夹层，无论其病因如何，都不是惰性的，可以保留特定的蛋白酶。(c)2009 Elsevier Inc. All rights reserved.
Histopathological alterations in human aneurysms and dissections of the thoracic ascending aorta include areas of mucoid degeneration within the medial layer, colocalized with areas of cell disappearance and disruption of extracellular matrix elastic and collagen fibers. We studied the presence of matrix metalloproteinases in relation to their capacity to diffuse through the tissue or to be retained in areas of mucoid degeneration in aneurysms and dissections of the ascending aorta. Ascending aortas from 9 controls, 33 patients with aneurysms, and 14 with acute dissections, all collected at surgery, were analyzed. The morphological aspect was similar whatever the etiology or phenotypic expression of the pathological aortas, involving areas of extracellular matrix breakdown and cell rarefaction associated with mucoid degeneration. Release of proMMP-2, constitutively expressed by smooth muscle cells, was not different between controls and aneurysmal aortas, whereas the aneurysmal aortas released more of the active form. Release of pro and active MMP-9 was also similar between controls and aneurysmal aortas. Immunohistochemical staining of MMP-2 and MMP-9 was weak in both control and pathological aortas. In contrast, released MMP-7 (matrilysin) and MMP-3 (stromelysin-1) could not be detected in conditioned media but were present in tissue extracts with no detectable quantitative difference between controls and pathological aortas. Immunohistochemical staining of MMP-7 and MMP-3 revealed their retention in areas of mucoid degeneration, and semiquantitative evaluation of immunostaining showed more MMP-7 in pathological aortas than in controls. In conclusion, areas of mucoid degeneration, the hallmark of aneurysms, and dissections of thoracic ascending aortas, whatever their etiology, are not inert and can retain specific proteases. (c) 2009 Elsevier Inc. All rights reserved.