Placenta- versus bone-marrow-derived mesenchymal cells for the repair of segmental bone defects in a rabbit model
Placenta- versus bone-marrow-derived mesenchymal cells for the repair of segmental bone defects in a rabbit model
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DOI：
10.1111/j.1742-4658.2012.08625.x
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发表时间：
2012-07-01
期刊：
影响因子：
5.4
通讯作者：
Xie, Hui-Qi
中科院分区：
文献类型：
--
作者：
Fan, Zhao-Xin;Lu, Yao;Xie, Hui-Qi
以骨髓间充质干细胞(BMSCs)为载体构建的组织工程化骨在动物实验和临床实验中均取得了良好的骨修复效果。然而，这受到骨髓间充质干细胞来源和数量的限制。我们探索了由胎盘来源的间充质干细胞(PMSCs)构建的TEBS，并比较了它们与BMSCs构建的TEBS修复临界大小的节段性骨膜缺损的效果。采用梯度离心法和体外单层培养法从兔胎盘分离PMSCs，从新生兔后肢骨髓分离BMSCs。原代培养的PMSCs和BMSCs均呈纺锤形。免疫细胞化学检测两种细胞CD44、CD105阳性，CD34、CD40L阴性，证实为间充质干细胞。将BrdU标记的PMSCs和BMSCs分别与生物衍生骨材料共培养，体外构建TEBS。手术造成24只兔桡骨临界大小的节段性骨膜缺损。用PMSCs和BMSCs构建的TEBS修复缺损区。结果表明，PMSCs和BMSCs构建的TEBS均能多点修复骨膜缺损区。放射学、组织学、免疫组织化学、碱性磷酸酶活性、骨钙素测定及生物力学性能检测发现，移植后2、4、8、12周两组间差异无统计学意义(P>；0.05)。综上所述，我们的结果表明，PMSCs具有与BMSCs相似的生物学特性和成骨能力，可以作为TEBS的一种新的种子细胞来源。
Tissue-engineered bones (TEBs) constructed with bone-marrow-derived mesenchymal stem cells (BMSCs) seeded on biomaterial scaffolds have achieved good results for bone defect repair in both animal experiments and clinical trials. This has been limited, however, by the source and quantity of BMSCs. We here explored TEBs constructed by placenta-derived mesenchymal stem cells (PMSCs) and compared their effect for the repair of critical-sized segmental osteoperiosteal defects with TEBs constructed with BMSCs. PMSCs were isolated from rabbit placenta by gradient centrifugation and in vitro monolayer culturing, and BMSCs were isolated from the hindlimb bone marrow of newborn rabbit. Primary cultured PMSCs and BMSCs were uniformly in a spindle shape. Immunocytochemistry indicated that both types of cells are positive for CD44 and CD105, and negative for CD34 and CD40L, confirming that they are mesenchymal stem cells. BrdU-labeled PMSCs and BMSCs were respectively co-cultured with bio-derived bone materials to construct TEBs in vitro. Critical-sized segmental osteoperiosteal defects of radii were created in 24 rabbits by surgery. The defects were repaired with TEBs constructed with PMSCs and BMSCs. The results showed that TEBs constructed by both PMSCs and BMSCs could repair the osteoperiosteal defects in a multipoint manner. Measurement of radiography, histology, immunohistochemistry, alkaline phosphatase activity, osteocalcin assaying and biomechanical properties have found no significant difference between the two groups at 2, 4, 8 and 12 weeks after the transplantation (P > 0.05). Taken together, our results indicate that PMSCs have similar biological characteristics and osteogenic capacity to BMSCs and can be used as a new source of seeding cells for TEBs.