Diverse steroidogenic pathways in the marine alga Aurantiochytrium
Diverse steroidogenic pathways in the marine alga Aurantiochytrium
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DOI：
10.1007/s10811-020-02078-4
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发表时间：
2020-03
影响因子：
3.3
通讯作者：
Masaki Yoshida;Motohide Ioki;H. Matsuura;A. Hashimoto;K. Kaya;N. Nakajima;M. Watanabe
中科院分区：
文献类型：
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作者：
Masaki Yoshida;Motohide Ioki;H. Matsuura;A. Hashimoto;K. Kaya;N. Nakajima;M. Watanabe
异养藻类橙色壶菌积累了大量的角鲨烯，这是一个关键的前体生物合成的甾醇和类固醇。然而，藻类的后角鲨烯甾醇途径一直是个谜。利用核磁共振化学方法，结合对橙黄壶菌基因组的计算机分析，我们鉴定了7种甾醇，包括新的衍生物，并预测了多种甾醇代谢途径。基于已知的甾醇在真核生物中的分布、系统发育和我们的研究结果，我们认为，橙花壶菌可能具有光合和非光合两个谱系的多种甾醇代谢途径，编码相关酶的基因高度增殖，可能具有发达的甾醇代谢途径网络。
The heterotrophic algaAurantiochytriumaccumulates a high amount of squalene, which is a key precursor to the biosynthesis of sterols and steroids. However, post-squalene sterol pathways of the algae have been enigmatic. Using NMR-based chemical approaches complemented by in silico analysis ofAurantiochytriumgenome, we identified 7 sterols including novel derivatives and predicted multiple sterol metabolic pathways. Based on known sterol distribution among eukaryotes, phylogeny, and our findings, we suggest thatAurantiochytriumcould possess multiple sterol metabolic pathways of both photosynthetic and non-photosynthetic lineages with highly proliferated genes encoding the associated enzymes.Aurantiochytriumpossibly possesses well-developed networks of sterol metabolic pathways.