Detection of melatonin and homocysteine simultaneously in ulcerative colitis
Detection of melatonin and homocysteine simultaneously in ulcerative colitis
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DOI：
10.1016/j.cca.2011.06.036
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发表时间：
2012-01-18
影响因子：
5
通讯作者：
Liu, Xiaochang
中科院分区：
文献类型：
--
作者：
Chen, Moli;Mei, Qiao;Liu, Xiaochang
背景：氧化应激可能是导致炎症性肠病（IBD）特征的组织损伤的一个主要因素。同型半胱氨酸（HCY）可能对溃疡性结肠炎（UC）患者的结肠组织造成氧化损伤，补充褪黑激素（MLT）可以减轻HCY在体外和体内引起的氧化损伤。本研究旨在同时测定UC患者血浆HCY和MLT水平。方法：收集112例UC患者和110名健康对照者（HC）的临床资料。采用HPLC-FD法检测血浆HCY、MLT水平。采用ELISA法检测血浆叶酸、维生素B（12）（VitB（12））水平。结果：UC患者血浆HCY水平显着高于HC患者（1127 726 μmol/L vs. 8.19±4.81 μmol/L，P=0.000）。 UC患者血浆MLT水平显着低于HC患者（49.06±31.40 pg/ml vs 64.28±41.16 pg/ml，P=0.008）。 UC患者血浆叶酸、VitB12水平低于HC患者（7.64±1.95nmol/L比9.14±123nmol/L、108.64±32.22pmol/L比112.64±33.33pmol/L，P<0.05）。 UC患者血浆MLT水平升高，血浆HCY水平降低，但血浆MLT与HCY水平的相关性无统计学意义（P>0.05）。结论：UC患者血浆HCY水平升高，血浆MLT水平降低。皇冠版权所有 (C) 2011 由 Elsevier B.V. 出版。保留所有权利。
Background: Oxidative stress could be a major contributing factor to the tissue injury that characterize inflammatory bowel disease (IBD). Homocysteine (HCY) could cause oxidative damage to the colon tissue in ulcerative colitis (UC) patients, melatonin (MLT) supplementation could reduce oxidative damage that caused by HCY in vitro and in vivo. In this study, we aimed to determine the levels of plasma HCY and MLT simultaneously in UC patients.Methods: Collected the clinical data of 112 UC patients and 110 healthy controls (HC). The levels of plasma HCY and MLT were detected by HPLC-FD method. The levels of plasma folate, vitamin B(12) (VitB(12)) were detected by ELISA method.Results: The levels of plasma HCY in UC patients were significantly higher than that in HC (1127 726 mu mol/L vs. 8.19 +/- 4.81 mu mol/L, P=0.000). The levels of plasma MLT in UC patients were significantly lower than that in HC (49.06 +/- 31.40 pg/ml vs. 64.28 +/- 41.16 pg/ml, P=0.008). The levels of plasma folate and VitB12 in UC patients were lower than that in HC (7.64 +/- 1.95 nmol/L vs. 9.14 +/- 123 nmol/L, 108.64 +/- 32.22 pmol/L vs. 112.64 +/- 33.33 pmol/L, P0.05). The change of increasing levels of plasma MLT but decreasing levels of plasma HCY was shown in UC patients, however, the association between the levels of plasma MLT and HCY were not statistically significant (P>0.05).Conclusions: The levels of plasma HCY were increased whereas the levels of plasma MLT were decreased in UC patients. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.